Affiliation:
1. Department of Rehabilitation Medicine, Huashan Hospital Fudan University Shanghai China
2. Behavioral and Cognitive Neuroscience Center Institute of Science and Technology for Brain‐Inspired Intelligence, Fudan University Shanghai China
3. Departments of Molecular Biophysics and Biochemistry and Neuroscience Yale University New Haven Connecticut USA
Abstract
AbstractBackgroundRepetitive transcranial magnetic stimulation (rTMS) is an effective therapy in post‐stroke motor recovery. However, the underlying mechanisms of rTMS regulates long‐lasting changes with synaptic transmission and glutamate receptors function (including AMPARs or NMDARs) remains unclear.MethodsMice were received 10‐Hz rTMS treatment once daily on the third day after photothrombotic (PT) stroke for 18 days. Motor behaviors and the Western blot were used to evaluate the therapeutic efficacy of 10‐Hz rTMS in the mice with PT model. Moreover, we used wild‐type (WT) and NEX‐α3−/− mice to further explore the 10‐Hz rTMS effect.ResultsWe found that 10‐Hz rTMS improved the post‐stroke motor performance in the PT mice. Moreover, the levels of AMPAR, vGlut1, and integrin α3 in the peri‐infarct were significantly increased in the rTMS group. In contrast, 10‐Hz rTMS did not induce these aforementioned effects in NEX‐α3−/− mice. The amplitude of AMPAR‐mediated miniature excitatory postsynaptic currents (EPSCs) and evoked EPSCs was increased in the WT + rTMS group, but did not change in NEX‐α3−/− mice with rTMS.ConclusionsIn this study, 10‐Hz rTMS improved the glutamatergic synaptic transmission in the peri‐infract cortex through effects on integrin α3 and AMPARs, which resulted in motor function recovery after stroke.
Funder
National Natural Science Foundation of China
Subject
Pharmacology (medical),Physiology (medical),Psychiatry and Mental health,Pharmacology