Skeletal muscle‐enriched miRNAs are highly unstable in vivo and may be regulated in a Dicer‐independent manner

Abstract

MicroRNAs (miRNAs) are small noncoding RNAs that control essential cellular processes. For several decades, the molecular mechanisms underlying the functions and biogenesis of miRNAs have been clarified, whereas the molecular dynamics of miRNAs are poorly understood. We recently found that muscle‐enriched miRNAs were reduced by only 20 ~ 50% in the skeletal muscles even 4 weeks after the suppression of miRNA processing through an inducible depletion of Dicer1 gene. These data suggest that miRNAs are stably expressed in skeletal muscle. In this study, we investigated the half‐lives of those miRNAs in adult skeletal muscle with an in vivo metabolic labeling strategy and a genetic mouse model. In contrast to the hypothesis, in vivo metabolic labeling revealed that the half‐lives of skeletal‐muscle‐enriched miRNAs were approximately 11–20 h. Furthermore, the levels of mature miR‐23a decreased rapidly in the skeletal muscle of mice lacking miR‐23 clusters in a tamoxifen‐inducible manner. These data suggest that skeletal‐muscle‐enriched miRNAs are not highly stable in vivo. We also observed that the transfer of miR‐150 into Dicer1‐deficient muscle increased the miR‐150 level to the same as that in control muscle. Taken together, our data demonstrate that miRNAs are degraded within a few days in adult skeletal muscle and that a Dicer‐independent biogenetic pathway may produce mature miRNAs.