Affiliation:
1. Department of Microbiology and Immunobiology Harvard Medical School Boston MA 02115 USA
2. Department of Biological Chemistry and Molecular Pharmacology Harvard Medical School Boston MA 02115 USA
Abstract
SummaryWhile soil‐dwelling actinomycetes are renowned for secreting natural products, little is known about the roles of these molecules in mediating actinomycete interactions. In a previous co‐culture screen, we found that one actinomycete, Amycolatopsis sp. AA4, inhibited aerial hyphae formation in adjacent colonies of Streptomyces coelicolor. A siderophore, amychelin, mediated this developmental arrest. Here we present genetic evidence that confirms the role of the amc locus in the production of amychelin and in the inhibition of S. coelicolor development. We further characterize the Amycolatopsis sp. AA4 – S. coelicolor interaction by examining expression of developmental and iron acquisition genes over time in co‐culture. Manipulation of iron availability and/or growth near Amycolatopsis sp. AA4 led to alterations in expression of the critical developmental gene bldN, and other key downstream genes in the S. coelicolor transcriptional cascade. In Amycolatopsis sp. AA4, siderophore genes were downregulated when grown near S. coelicolor, leading us to find that deferrioxamine E, produced by S. coelicolor, could be readily utilized by Amycolatopsis sp. AA4. Collectively these results suggest that competition for iron via siderophore piracy and species‐specific siderophores can alter patterns of gene expression and morphological differentiation during actinomycete interactions.
Funder
Broad Institute
National Institutes of Health
NIH Pathway to Independence Award
NIH Postdoctoral Fellow
Cited by
153 articles.
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