Affiliation:
1. Department of Pathology and Applied Neurobiology Kyoto Prefectural University of Medicine Graduate School of Medical Science Kyoto Japan
Abstract
AbstractPrimary cilia on neural stem/progenitor cells (NSPCs) play an important role in determining cell fate, although the regulatory mechanisms involved in the ciliogenesis remain largely unknown. In this study, we analyzed the effect of the leukemia inhibitory factor (LIF) for the primary cilia in immortalized human NSPCs. LIF withdrawal elongated the primary cilia length, whereas the addition of LIF shortened it. Microarray gene expression analysis revealed that differentially expressed genes (DEGs) associated with LIF treatment were related with the multiple cytokine signaling pathways. Among the DEGs, C‐C motif chemokine 2 (CCL2) had the highest ranking and its increase in the protein concentration in the NSPCs‐conditioned medium after the LIF treatment was confirmed by ELISA. Interestingly, we found that CCL2 was a negative regulator of cilium length, and LIF‐induced shortening of primary cilia was antagonized by CCL2‐specific antibody, suggesting that LIF could influence cilia length via upregulating CCL2. The shortening effect of LIF and CCL2 on primary cilia was also observed in SH‐SY5Y cells. The results of the study suggested that the LIF‐CCL2 axis may well be a regulator of NSPCs and its primary cilia length, which could affect multiple cellular processes, including NSPC proliferation and differentiation.