Affiliation:
1. Institute of Environmental Engineering National Sun Yat‐sen University Kaohsiung Taiwan
2. Department of Chemical and Materials Engineering National Central University Taoyuan Taiwan
3. Department of Photonics National Sun Yat‐Sen University Kaohsiung Taiwan
4. Department of Dermatology Kaohsiung Chang Gung Memorial Hospital Kaohsiung Taiwan
5. Department of Dermatology Chang Gung University College of Medicine Taoyuan Taiwan
Abstract
AbstractPurposeCurrent skin imaging modalities, including optical, electron, and confocal microscopy, mostly require tissue fixations that could damage proteins and biological molecules. Live tissue or cell imaging such as ultrasonography and optical coherent microscope may not adequately measure the dynamic spectroscopical changes. Raman spectroscopy has been adopted for skin imaging in vivo, mostly for skin cancer imaging. However, whether the epidermal and dermal thickening in skin could be measured and distinguished by conventional Ramen spectroscopy or the surface‐enhanced Raman scattering (SERS), a rapid and label‐free method for noninvasive measurement remains unknown.MethodsHuman skin sections from patients of atopic dermatitis and keloid, which represent epidermal and dermal thickening, respectively, were measured by conventional Ramen spectroscopy. In mice, skin sections from imiquimod (IMQ)‐ and bleomycin (BLE)‐treated mice, which reflect the epidermal and dermal thickening, respectively, were measured by SERS, that incorporates gold nanoparticles to generate surface plasma and enhance Raman signals.ResultsConventional Ramen spectroscopy failed to consistently show the Raman shift in human samples among the different groups. SERS successfully revealed a prominent peak around 1300 cm−1 in the IMQ‐treated skin; and two significant peaks around 1100 and 1300 cm−1 in BLE‐treated group. Further quantitative analysis showed 1100 cm−1 peak was significantly accentuated in the BLE‐treated skin than that in control skin. SERS identified in vitro a similar 1100 cm−1 peak in solutions of collagen, the major dermal biological molecules.ConclusionSERS distinguishes the epidermal or dermal thickening in mouse skin with rapid and label‐free measures. A prominent 1100 cm−1 SERS peak in the BLE‐treated skin may result from collagen. SERS might help precision diagnosis in the future.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献