Small-volume vitrification for human spermatozoa in the absence of cryoprotectants by using Cryotop
Author:
Affiliation:
1. Reproductive Medicine Center; Taihe Hospital; Hubei University of Medicine; Shiyan Hubei China
2. Department of Reproductive Endocrinology; Women's Hospital; Zhejiang University School of Medicine; Hangzhou Zhejiang China
Funder
China Natural Science Fundation
Zhejiang Medicines & Health Science and Technology Pro
Publisher
Wiley
Subject
Urology,Endocrinology,General Medicine
Link
http://onlinelibrary.wiley.com/wol1/doi/10.1111/and.12320/fullpdf
Reference24 articles.
1. Techniques for cryopreservation of individual or small numbers of human spermatozoa: a systematic review;AbdelHafez;Hum Reprod Update,2009
2. Reactive oxygen species and cryopreservation promote DNA fragmentation in equine spermatozoa;Baumber;J Androl,2003
3. Single spermatozoon freezing using Cryotop;Endo;J Mamm Ova Res,2011
4. Simple vitrification for small numbers of human spermatozoa;Endo;Reprod Biomed Online,2012a
5. Successful delivery derived from vitrified-warmed spermatozoa from a patient with nonobstructive azoospermia;Endo;Fertil Steril,2012b
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3. Ultra-Rapid Freezing Preserves Morphofunctional Integrity and Fertilizing Ability of Epididymal Cat Spermatozoa;Frontiers in Veterinary Science;2022-06-14
4. Single sperm vitrification with permeable cryoprotectant-free medium is more effective in patients with severe oligozoospermia and azoospermia;Cryobiology;2022-02
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