Cytotoxicity, biocompatibility and osteoinductive profile of an MTA‐hydrogel‐based cement: An in vitro and animal study

Author:

Martins Manoela Domingues12ORCID,Coelho Fernanda Hack3,Moreira Maria Stella4ORCID,Marques Márcia Martins5,Pilar Emily Ferreira Salles12,Palo Renato Miotto6,Silveira Felipe Martins17ORCID,Kopper Patrícia Maria Poli1ORCID

Affiliation:

1. Oral Pathology Department, School of Dentistry Universidade Federal do Rio Grande do Sul (UFRGS) Porto Alegre Brazil

2. Department of Experimental Pathology Hospital de Clínicas de Porto Alegre (HCPA) Porto Alegre Brazil

3. Graduate Program in Dentistry/Endodontics, School of Dentistry Universidade Federal do Rio Grande do Sul (UFRGS) Porto Alegre Brazil

4. Department of Stomatology A.C.Camargo Cancer Center São Paulo Brazil

5. Department of Dentistry, School of Dentistry Universidade de São Paulo (FOUSP) São Paulo Brazil

6. Master and Doctorate in Endodontics Universidade Estadual Paulista (UNESP) São Paulo Brazil

7. Molecular Pathology Area, School of Dentistry Universidad de la República (UDELAR) Montevideo Uruguay

Abstract

AbstractAimThis study aimed to evaluate the cytotoxicity, biocompatibility and osteoinductive profile of a mineral trioxide aggregate (MTA)‐hydrogel‐based material (MTA Flow) in comparison with MTA Angelus.MethodologyCell viability was evaluated in human periodontal ligament stem cells (hPDLSCs) using the methyl‐thiazol‐tetrazolium (MTT) colourimetric assay. Polyethylene tubes containing the tested materials and empty polyethylene tubes (control) were implanted in the subcutaneous tissue of Wistar rats. Cellular (lymphocyte infiltration) and extracellular events (ECM; collagen fibres) were analysed in histological sections. Immunohistochemical (collagen I, osteopontin, bone sialoprotein, bone morphogenetic protein4) analyses were also performed.ResultsAt 24, 48 and 72 h, all tested groups showed cell viability similar to control (p > .05). Regarding biocompatibility, all groups showed similar cellular events represented by a slight inflammatory reaction characterized by hyperaemia and a mild lymphocytic inflammatory infiltrate. The analysis of lymphocytes during the time showed a decrease in these cells in the control group and a significant interaction between MTA Angelus and control (p < .001), with MTA Angelus showing a more extensive inflammatory infiltrate. Regarding fibres, an increase in content was observed in all groups during the experimental time (7, 30 and 60 days), however, no difference was detected among the experimental groups (p = .063). After 60 days, the immunoexpression of bone matrix proteins in the MTA Flow group was similar to or higher than that observed in the MTA Angelus and in the control group.ConclusionsMTA Flow showed a non‐cytotoxic behaviour, biocompatibility and ability to stimulate tissue mineralization.

Funder

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

Publisher

Wiley

Subject

General Dentistry

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