Salivary microbiomes vary among orthodontic appliances and associate with clinical periodontal parameters

Abstract

AbstractObjectiveTo investigate the salivary bacterial communities during the first 6‐month orthodontic treatment with Clear Aligners (CA) and Fixed Appliances (FA), and its correlation with clinical periodontal parameters.Materials and MethodsSaliva and periodontal parameters were sampled from individuals wearing CA or FA before treatment (T0), and after 3‐ (T3) and 6‐month (T6) treatments. Salivary bacterial communities characterized based on the 16S rRNA V3‐V4 region were compared between FA and CA and correlated with clinical periodontal parameters.ResultsProbing Depth (PD) significantly increased at T6 in the FA group versus T0, whereas it remained stable in the CA group. The Shannon and Pielou indices were significantly higher in the FA group and significantly positively correlated with periodontal inflammation parameters. β‐diversity analysis revealed distinct communities between the FA group and CA group at T6. The relative abundances of 3 genera and 15 species were significantly higher in the FA group. Among the above appliance‐type related taxa, bacterial genera Selenomonas, Stomatobaculum, Olsenella and Faecalicoccus and bacterial species Selenomonas_sputigena, Dialister_invisus, Olsenella_profus, Prevotella_buccae, Cryptobacterium_curtum and Clostridium_spiroforme were significantly positively associated with periodontal parameters.ConclusionsOrthodontic treatments trigger appliance‐related salivary bacterial communities, highlighting the importance of developing appliance–orientated periodontal strategies during orthodontic treatments. Salivary bacterial communities harboured by patients wearing FA possess higher bacterial parameters which were associated with increasing PD, PI and Gingival Index.