Affiliation:
1. Department of Life Sciences and Biotechnology – Section of Biology and Evolution University of Ferrara Ferrara Italy
2. Research Centre for the Study of Periodontal and Peri‐Implant Diseases University of Ferrara Ferrara Italy
3. Operative Unit of Dentistry Azienda Unità Sanitaria Locale (A.U.S.L.) Ferrara Italy
4. Department of Life Sciences and Biotechnology – Section of Pathology and Applied Microbiology University of Ferrara Ferrara Italy
Abstract
AbstractIncreasing evidence support the association between the oral microbiome and human systemic diseases. This association may be attributed to the ability of many oral microbes to influence the inflammatory microenvironment. Herein, we focused our attention on the bidirectional relationship between periodontitis and type 2 diabetes using high‐resolution whole metagenomic shotgun analysis to explore the composition and functional profile of the subgingival microbiome in diabetics and non‐diabetics subjects with different periodontal conditions.In the present study, the abundance of metabolic pathways encoded by oral microbes was reconstructed from the metagenome, and we identified a set of dysregulated metabolic pathways significantly enriched in the periodontitis and/or diabetic patients. These pathways were mainly involved in branched and aromatic amino acids metabolism, fatty acid biosynthesis and adipocytokine signaling pathways, ferroptosis and iron homeostasis, nucleotide metabolism, and finally in the peptidoglycan and lipopolysaccharides synthesis.Overall, the results of the present study provide evidence in favor of the hypothesis that during the primary inflammatory challenge, regardless of whether it is induced by periodontitis or diabetes, endotoxemia and/or the release of inflammatory cytokines cause a change in precursor and/or in circulating innate immune cells. Dysbiosis and inflammation, also via oral–gut microbiome axis or adipose tissue, reduce the efficacy of the host immune response, while fueling inflammation and can induce that metabolic/epigenetic reprogramming of chromatin accessibility of genes related to the immune response.Moreover, the presence of an enhanced ferroptosis and an imbalance in purine/pyrimidine metabolism provides new insights into the role of ferroptotic death in this comorbidity.
Subject
Microbiology (medical),General Dentistry,Immunology,Microbiology
Reference90 articles.
1. Targeting Nrf2 to Suppress Ferroptosis and Mitochondrial Dysfunction in Neurodegeneration
2. Microbiota, cirrhosis, and the emerging oral-gut-liver axis
3. Andrews S.(2010).FastQC: a quality control tool for high throughput sequence data.http://www.bioinformatics.babraham.ac.uk/projects/fastqc/