Affiliation:
1. Reproductive Medicine Center Renmin Hospital of Wuhan University Wuhan Hubei China
2. Shenzhen Key Laboratory of Reproductive Immunology for Peri‐implantation Shenzhen Zhongshan Institute for Reproduction and Genetics Fertility Center Shenzhen Zhongshan Urology Hospital Shenzhen Guangdong China
3. Guangdong Engineering Technology Research Center of Reproductive Immunology for Peri‐implantation Shenzhen Guangdong China
Abstract
AbstractProblemRM is a common clinical disease in reproduction, affecting approximately 1%–3% of women worldwide. Previous studies have shown the role of peripheral blood γδ‐T cells during physiological pregnancy. However, the relationship between the immune status of peripheral blood γδ‐T cells and RM is still not well defined.Method of StudyIn this study, mid‐luteal peripheral blood from 51 RM patients and 40 healthy women was collected to determine the immune status of γδ‐T cells. The percentage of peripheral blood γδ‐T cells, and the molecules mediating their toxic potential, including cytotoxic granules (perforin, granzyme B, and granulysin) and receptors (NKG2D, CD158a, and CD158b), were detected by flow cytometry.ResultsCompared to healthy control, an increase in the proportion of total CD3+ T cells in lymphocytes and a decrease in the ratio of γδ‐T cells to CD3+ T cells were observed in patients with RM. The percentages of granzyme B+ γδ‐T cells and CD158a+ γδ‐T cells in total γδ‐T cells or lymphocytes were significantly increased in patients with RM, compared with healthy control. Conversely, CD158b+ γδ‐T cells in total γδ‐T cells or lymphocytes were significantly decreased in the RM group.ConclusionIncreased peripheral blood γδ‐T cell with high toxic potential was associated with RM.
Funder
National Natural Science Foundation of China
Subject
Obstetrics and Gynecology,Reproductive Medicine,Immunology,Immunology and Allergy,Obstetrics and Gynecology,Immunology
Cited by
1 articles.
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