Author:
WOOD NANCY,BASHIR HELEN,GREALLY J.,AMOS D. B.,YUNIS E. J.
Abstract
A simple method of freezing lymphocytes requiring a cryoprotective medium consisting of glucose, MEM, and dimethylsulfoxide (DMSO) and an ultra‐low freezer to freeze and store the lymphocytes is described. The thawed lymphocytes following 2 weeks' to 5 months' storage were found to be useful for HL—A antibody screening, HL—A typing, and PHA stimulation.
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