Calcium‐dependent contractile response of arterial smooth muscle to a jellyfish toxin (pCrTX: Carybdea rastonii)

Author:

Azuma Hiroshi,Ishikawa Masayuki,Nakajima Terumi,Satoh Akihiko,Sekizaki Satomi

Abstract

The purpose of the present experiments was to investigate the pharmacological mechanisms of the vasoconstriction caused by the toxin (pCrTX) which had been partially purified from the tentacles of the jellyfish Carybdea rastonii (‘Andonkurage’). pCrTX (0.1 to 10 μg ml−1) produced a tonic contraction of rabbit aortic strips, which was nearly abolished in Ca2+‐free medium and was significantly reduced by verapamil or diltiazem. pCrTX stimulated 45Ca2+‐influx and this effect was markedly attenuated by verapamil. pCrTX‐induced vasoconstriction was significantly attenuated by phentolamine, 6‐hydroxydopamine (6‐OHDA) and in low Na+‐medium, but not by bretylium, guanethidine, reserpinization or tetrodotoxin (TTX). pCrTX continuously and significantly increased the 3H‐efflux from [3H]‐noradrenaline preloaded aortic strips and this effect was completely inhibited by pretreatment with 6‐OHDA and in Ca2+‐free medium, but not by phentolamine, bretylium, guanethidine or TTX. A single exposure to pCrTX for 30 min greatly reduced the contractile responses to tyramine, nicotine and transmural electrical stimulation, but not those to noradrenaline or KCl. In addition, incorporation of [3H]‐noradrenaline was reduced. Pretreatments with chlorphenylamine or indomethacin failed to modify the contractile response to pCrTX. These results suggest that the pCrTX‐induced vasoconstriction is caused by a presynaptic action, releasing noradrenaline from the intramural adrenergic nerve terminals, and by a postsynaptic action, which consists at least in part of stimulation of the transmembrane calcium influx. Both pre‐ and postsynaptic actions depend on the external calcium concentration. The data further suggest that pCrTX damages the noradrenaline uptake and/or storage mechanisms without damaging postsynaptic contractile systems.

Publisher

Wiley

Reference23 articles.

1. Differential inhibitory effects of the ionophore RO2‐2985 (X537A) on contractile responses to potassium and histamine in coronary artery smooth muscle;Berner P.F.;J. Pharmac. exp. Ther.,1980

2. The Up‐and‐Down method with small samples;Brownlee K.A.;Am. Statist. Assoc. J.,1953

3. Effects of Chrysaora quinquecirrha (Sea Nettle) Toxin on the Rat Cardiovascular System

4. Further studies of toxic material from nematocysts of the cubomedusan Chironex fleckeri southcott

5. Actions of Chironex fleckeri toxins on cardiac transmembrane potentials

Cited by 30 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.7亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2025 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3