The effects of Fasciola hepatica recombinant proteins (peroxiredoxin and cathepsin L1) on Crohn's disease experimental model

Author:

Hasanpour Hamid12,Falak Reza34,Mokhtarian Kobra5,Sadeghi Fatemeh6,Masoumi Elham78,Asadollahi Parisa9,Badirzadeh Alireza10ORCID,Azami Sanaz Jafarpour11,Gholami Mohammad Davoodzadeh4,Pashangzadeh Salar4,Gharagozlou Mohammad Javad12,Naserifar Razi12,Mowlavi Gholamreza11

Affiliation:

1. Department of Parasitology and Mycology, School of Allied Medical Sciences Ilam University of Medical Sciences Ilam Iran

2. Zoonotic Diseases Research Center Ilam University of Medical Sciences Ilam Iran

3. Immunology Research Center Iran University of Medical Sciences Tehran Iran

4. Department of Immunology, School of Medicine Iran University of Medical Sciences Tehran Iran

5. Department of Parasitology and Mycology, School of Medicine Shahrekord University of Medical Sciences Shahrekord Iran

6. Department of Medical Epidemiology and Biostatistics Karolinska Institute Solna Sweden

7. Department of Radiation and Cellular Oncology University of Chicago Chicago Illinois USA

8. Ludwig Center for Metastasis Research University of Chicago Chicago Illinois USA

9. Department of Microbiology, Faculty of Medicine Ilam University of Medical Sciences Ilam Iran

10. Department of Parasitology and Mycology, School of Medicine Iran University of Medical Sciences Tehran Iran

11. Department of Parasitology and Mycology, School of Public Health Tehran University of Medical Sciences Tehran Iran

12. Department of Pathobiology, Faculty of Veterinary Medicine University of Tehran Tehran Iran

Abstract

AbstractThe immunomodulatory potential of the excretory‐secretory (E/S) proteins of the helminths has been shown in previous investigations. This study evaluated the effects of the recombinants and excretory‐secretory proteins of the Fasciola hepatica on induced colitis in Balb/c mice. The F. hepatica Recombinant proteins, Cathepsin L1 and Peroxiredoxin, and E/S proteins were intraperitoneally injected into the three mice groups as the case groups, while the control groups received PBS. Colitis was induced in mice by intraluminal administration of the 2, 4, 6‐Trinitrobenzenesulfonic acid solution (TNBS). After 8 h, the case groups received the second dosage of the treatments, and it was repeated 24 h later. The immunological, pathological, and macroscopic changes were evaluated 3 days after colitis induction. The macroscopic evaluation revealed significantly lower inflammatory scores in the mice treated with recombinant Peroxiredoxin (rPRX) and recombinant Cathepsin L1 (rCL1). Despite the macroscopic observation, the pathological finding was insignificant between the groups. IFN‐γ secretion was significantly lower in splenocytes of the groups that received rPRX, rCL1, and E/S than the controls. IL‐10 showed significantly higher levels in groups treated with rPRX and rCL1 than controls, whereas the level of IL‐4 was not statistically significant. Excretory‐secretory proteins of the F. hepatica showed immunomodulatory potency and the main effects observed in this study were through the reduction of inflammatory cytokine and inflammation manifestation as well as induction of anti‐inflammatory cytokines.

Publisher

Wiley

Subject

Immunology,Parasitology

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3