Characterization of anAvenaisolate ofMagnaporthe griseaand identification of a locus conditioning its specificity on oat

Abstract

An isolate of Magnaporthe grisea was collected from a blast lesion on oat in Brazil. Sequence analysis of the rDNA-ITS-2 region and DNA fingerprinting with repetitive elements revealed that the Avena isolate belongs to the "crop isolate group" and is similar to Triticum isolates. At high temperature (28°C), the Avena isolate caused severe disease symptoms on primary leaves of oat and wheat. When the temperature was decreased to 20°C, wheat leaves expressed resistance to the Avena isolate. Cytologically, this temperature-dependent resistance was associated with an increase in the incidences of papilla formation and a hypersensitive reaction. Pathogenicity tests with various plant species at 20°C revealed that the Avena isolate is exclusively parasitic on oat. To elucidate genetic mechanisms of this species-specific parasitism, the Avena isolate was crossed with a Triticum isolate and resulting F1progenies were subjected to pathogenicity tests on oat seedlings. In the F1population, avirulent and virulent cultures segregated in a 1:1 ratio, suggesting that the specific parasitism on oat is controlled by a single gene. This locus was designated as Pat1.Key words: Magnaporthe grisea, species-specific parasitism, oat, temperature sensitive.