Time of day, but not sleep restriction, affects markers of hemostasis following heavy exercise

Abstract

We sought to determine the effects of sleep restriction on markers of hemostasis the morning after an exercise session. Seven subjects performed evening exercise followed by an exercise session the next morning, both with and without sleep restriction. Evening exercise included a 20-min submaximal cycling trial (10 min at 50% maximal power (Wmax), 10 min at 60% Wmax), a 3-km cycling time trial, 60 min of cycling intervals, and 3 sets of leg press. Subsequent morning exercise was the same, excluding intervals and leg press. Blood samples were collected at rest and following the 20-min submaximal trial for factor VIII antigen, tissue plasminogen activator (tPA) activity, and plasminogen activator inhibitor-1 (PAI-1) activity. Sleep restriction had no effect on the variables. Factor VIII antigen was higher and tPA activity lower in the morning versus evening, respectively (P < 0.05). There were larger (P < 0.05) exercise responses for tPA activity in the evening (pre-exercise = 0.32 ± 0.14, postexercise = 1.89 ± 0.60 AU/mL) versus morning (pre-exercise = 0.27 ± 0.13 AU/mL, postexercise = 0.69 ± 0.18 AU/mL). PAI-1 exhibited lower (P < 0.05) responses in the evening (pre-exercise = 0.78 ± 0.26 AU/mL, postexercise = 0.69 ± 0.29 AU/mL) versus morning (pre-exercise = 7.06 ± 2.66, postexercise = 5.40 ± 2.31 AU/mL). Although a prothrombotic environment was observed the morning following an evening exercise session, it was not exacerbated by sleep restriction.