Abstract
Structured illumination microscopy (SIM) holds great promise for live cell imaging applications due to its potential to obtain multidimensional information such as intensity, spectrum and polarization (I, λ , p) at high spatial-temporal resolution, enabling the observation of more complex dynamic interactions between subcellular structures. However, the reconstruction results of polarized samples are prone to artifacts because all current SIM reconstruction frameworks use incomplete imaging models which neglect polarization modulation. Such polarization-related artifacts are especially prevalent for SIM reconstruction using a reduced number of raw images (RSIM) and severely undermine the ability of SIM to capture multi-dimensional information. Here, we report a new SIM reconstruction framework (PRSIM) that can recover multi-dimensional information (I, λ, p) using a reduced number of raw images. PRSIM adopts a complete imaging model that is versatile for normal and polarized samples and uses a frequency-domain iterative reconstruction algorithm for artifact-free super-resolution (SR) reconstruction. It can simultaneously obtain the SR spatial structure and polarization orientation of polarized samples using 6 raw SIM images and can perform SR reconstruction using 4 SIM images for normal samples. In addition, PRSIM has less spatial computational complexity and achieves reconstruction speeds tens of times higher than that of the state-of-the-art non-iterative RSIM, making it more suitable for large field-of-view imaging. Thus, PRSIM is expected to facilitate the development of SIM into an ultra-high-speed and multi-dimensional SR imaging tool.
Funder
CETC Haikang Group-Brain Inspired Computing Joint Research Center
National Key Research and Development Program of China
National Natural Science Foundation of China
Subject
Atomic and Molecular Physics, and Optics
Cited by
2 articles.
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