Affiliation:
1. Osaka Metropolitan University
2. Research Fellow of Japan Society for the Promotion of Science
Abstract
This study presents a depth map estimation of fluorescent objects in turbid media, such as biological tissue based on fluorescence observation by two-wavelength excitation and deep learning-based processing. A U-Net-based convolutional neural network is adapted for fluorophore depth maps from the ratiometric information of the two-wavelength excitation fluorescence. The proposed method offers depth map estimation from wide-field fluorescence images with rapid processing. The feasibility of the proposed method was demonstrated experimentally by estimating the depth map of protoporphyrin IX, a recognized cancer biomarker, at different depths within an optical phantom.
Funder
Japan Society for the Promotion of Science
Subject
Atomic and Molecular Physics, and Optics,Biotechnology