Rapid clearing and imaging of Mohs and melanoma surgical margins using a low-cost tissue processor

Author:

Huang Chi Z.ORCID,Montague Jenna E.1ORCID,Ching-Roa Vincent D.ORCID,Drage Michael G.2,Ibrahim Sherrif F.34,Giacomelli Michael G.ORCID

Affiliation:

1. University of Arizona

2. Mass General Brigham

3. Rochester Dermatologic Surgery

4. University of Rochester Medical Center

Abstract

Tissue clearing methods render biological tissues transparent while maintaining tissue structure, enabling visualization of entire tissues. Recent developments in tissue clearing have predominantly emphasized preserving intrinsic fluorescent proteins or aqueous-based tissue clearing and so typically involve complex procedures and long processing times. The utilization of tissue clearing protocols in standard of care histology settings has been less well explored, and protocols for rapid clearing of human tissue specimens are limited. This study presents a novel rapid clearing protocol and demonstrates a low-cost tissue processor for high volume rapid tissue clearing that can be intergraded into standard histology workflow. We demonstrate rapid clearing in dermatological specimens, including both nonmelanoma and melanoma excisions.

Funder

National Institutes of Health

Publisher

Optica Publishing Group

Subject

Atomic and Molecular Physics, and Optics,Biotechnology

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