High-throughput line-illumination Raman microscopy with multislit detection

Author:

Mochizuki Kentaro12,Kumamoto Yasuaki1ORCID,Maeda Shunsuke1,Tanuma Masato3,Kasai Atsushi3,Takemura Masashi2,Harada Yoshinori2,Hashimoto Hitoshi34,Tanaka Hideo2,Smith Nicholas Isaac3,Fujita Katsumasa125ORCID

Affiliation:

1. Osaka University,

2. Kyoto Prefectural University of Medicine

3. Osaka University

4. Osaka University, Kanazawa University, Hamamatsu University School of Medicine, Chiba University and University of Fukui

5. AIST-Osaka University, Osaka University

Abstract

Raman microscopy is an emerging tool for molecular imaging and analysis of living samples. Use of Raman microscopy in life sciences is, however, still limited because of its slow measurement speed for spectral imaging and analysis. We developed a multiline-illumination Raman microscope to achieve ultrafast Raman spectral imaging. A spectrophotometer equipped with a periodic array of confocal slits detects Raman spectra from a sample irradiated by multiple line illuminations. A comb-like Raman hyperspectral image is formed on a two-dimensional detector in the spectrophotometer, and a hyperspectral Raman image is acquired by scanning the sample with multiline illumination array. By irradiating a sample with 21 simultaneous illumination lines, we achieved high-throughput Raman hyperspectral imaging of mouse brain tissue, acquiring 1108800 spectra in 11.4 min. We also measured mouse kidney and liver tissue as well as conducted label-free live-cell molecular imaging. The ultrafast Raman hyperspectral imaging enabled by the presented technique will expand the possible applications of Raman microscopy in biological and medical fields.

Funder

Japan Science and Technology Agency

Core Research for Evolutional Science and Technology

Japan Agency for Medical Research and Development

Japan Society for the Promotion of Science

Nakatani Foundation for Advancement of Measuring Technologies in Biomedical Engineering

Publisher

Optica Publishing Group

Subject

Atomic and Molecular Physics, and Optics,Biotechnology

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3