Conditioned medium from human dental pulp stem cells treats spinal cord injury by inhibiting microglial pyroptosis-Reference-Cited by-同舟云学术

Conditioned medium from human dental pulp stem cells treats spinal cord injury by inhibiting microglial pyroptosis

Published:2023-09-22 Issue:5 Volume:19 Page:1105-1111
ISSN:1673-5374
Container-title:Neural Regeneration Research
language:en
Short-container-title:

Author:

Liu Tao¹^ORCID,Ma Ziqian¹,Liu Liang¹,Pei Yilun¹,Wu Qichao²,Xu Songjie¹,Liu Yadong¹,Ding Nan³,Guan Yun⁴⁵,Zhang Yan¹,Chen Xueming¹^ORCID

Affiliation:

1. Department of Orthopedic Surgery, Beijing Luhe Hospital, Capital Medical University, Beijing, China

2. Department of Orthopedic Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China

3. Department of Stomatology, Beijing Luhe Hospital, Capital Medical University, Beijing, China

4. Department of Anesthesiology and Critical Care Medicine, School of Medicine, Johns Hopkins University, Baltimore, MD, USA

5. Department of Neurological Surgery, School of Medicine, Johns Hopkins University, Baltimore, MD, USA

Abstract

AbstractJOURNAL/nrgr/04.03/01300535-202405000-00041/inline-graphic1/v/2023-09-28T063346Z/r/image-tiffHuman dental pulp stem cell transplantation has been shown to be an effective therapeutic strategy for spinal cord injury. However, whether the human dental pulp stem cell secretome can contribute to functional recovery after spinal cord injury remains unclear. In the present study, we established a rat model of spinal cord injury based on impact injury from a dropped weight and then intraperitoneally injected the rats with conditioned medium from human dental pulp stem cells. We found that the conditioned medium effectively promoted the recovery of sensory and motor functions in rats with spinal cord injury, decreased expression of the microglial pyroptosis markers NLRP3, GSDMD, caspase-1, and interleukin-1β, promoted axonal and myelin regeneration, and inhibited the formation of glial scars. In addition, in a lipopolysaccharide-induced BV2 microglia model, conditioned medium from human dental pulp stem cells protected cells from pyroptosis by inhibiting the NLRP3/caspase-1/interleukin-1β pathway. These results indicate that conditioned medium from human dental pulp stem cells can reduce microglial pyroptosis by inhibiting the NLRP3/caspase-1/interleukin-1β pathway, thereby promoting the recovery of neurological function after spinal cord injury. Therefore, conditioned medium from human dental pulp stem cells may become an alternative therapy for spinal cord injury.

Publisher

Medknow

Subject

Developmental Neuroscience

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