Recombinant Cas9 protein production in an endotoxin-free system and evaluation with editing the BCL11A gene in human cells
Author:
Funder
Mahidol University
National Science and Technology Development Agency
Publisher
Elsevier BV
Subject
Biotechnology
Reference37 articles.
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2. In vivo genome editing using Staphylococcus aureus Cas9;Ran;Nature,2015
3. Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in C57BL/6 zygotes;Chu;BMC Biotechnol.,2016
4. Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins;Kim;Genome Res.,2014
5. In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system;Maddalo;Nature,2014
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2. Optimizing Recombinant Cas9 Expression: Insights from E. coli BL21(DE3) Strains for Enhanced Protein Purification and Genome Editing;Biomedicines;2024-05-31
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