Single-round QuikChange PCR for engineering multiple site-directed mutations in plasmid DNA-Reference-Cited by-同舟云学术

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Single-round QuikChange PCR for engineering multiple site-directed mutations in plasmid DNA

Published:2024-11 Issue: Volume:694 Page:115621
ISSN:0003-2697
Container-title:Analytical Biochemistry
language:en
Short-container-title:Analytical Biochemistry

Author:

Li Yunxiang^ORCID,Pinones Mileina^ORCID,Breeland Alexis,Jiang Peilin

Funder

Welch Foundation

Publisher

Elsevier BV

Reference16 articles.

1. Multichange isothermal mutagenesis: a New strategy for multiple site-directed mutations in plasmid DNA;Mitchell;ACS Synth. Biol.,2013

2. An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol;Liu;BMC Biotechnol.,2008

3. Simple and efficient site-directed mutagenesis using two single-primer reactions in parallel to generate mutants for protein structure-function studies;Edelheit;BMC Biotechnol.,2009

4. Two-stage PCR protocol allowing introduction of multiple mutations, deletions and insertions using QuikChangeTM site-directed mutagenesis;Wang;Biotechniques,1999

5. Creating randomized amino acid libraries with the QuikChange® Multi site-directed mutagenesis kit;Hogrefe;Biotechniques,2002

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