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2. Ethanolic extract of the solid culture of the fungus KGT142 was partitioned between BuOH and H2O, and the BuOH-soluble portion was subjected to a column of SiO2 eluted with CHCl3/MeOH stepwise. The active fractions were combined and chromatographed on ODS flash column by increasing the ratio of MeOH to water. The active eluate, 90% aq. MeOH fraction, was further purified with ODS–TLC developed with 90% aq. MeOH, followed by HPLC (ODS column, 62% aq. MeCN, 3.0 ml/min) to give peptaivirins.
3. Kubo, S.; Ikeda, T.; Imaizumi, S.; Takanami, Y.; Mikami, Y. Ann. Phytopath. Soc. Jpn. 1990, 56, 481–487.