Author:
Visagie C.M.,Hirooka Y.,Tanney J.B.,Whitfield E.,Mwange K.,Meijer M.,Amend A.S.,Seifert K.A.,Samson R.A.
Abstract
As part of a worldwide survey of the indoor mycobiota, dust was collected from nine countries. Analyses of dust samples included the culture-dependent dilution-to-extinction method and the culture-independent 454-pyrosequencing. Of the 7 904 isolates, 2 717 isolates were
identified as belonging to Aspergillus, Penicillium and Talaromyces. The aim of this study was to identify isolates to species level and describe the new species found. Secondly, we wanted to create a reliable reference sequence database to be used for next-generation
sequencing projects. Isolates represented 59 Aspergillus species, including eight undescribed species, 49 Penicillium species of which seven were undescribed and 18 Talaromyces species including three described here as new. In total, 568 ITS barcodes were generated, and
391 β-tubulin and 507 calmodulin sequences, which serve as alternative identification markers.
Funder
Alfred P. Sloan Foundation
Publisher
Westerdijk Fungal Biodiversity Institute
Subject
Agricultural and Biological Sciences (miscellaneous)
Cited by
204 articles.
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