Total protein staining is superior to classical or tissue-specific protein staining for standardization of protein biomarkers in heterogeneous tissue samples-Reference-Cited by-同舟云学术

Total protein staining is superior to classical or tissue-specific protein staining for standardization of protein biomarkers in heterogeneous tissue samples

Published:2020-06 Issue: Volume:19 Page:100641
ISSN:2452-0144
Container-title:Gene Reports
language:en
Short-container-title:Gene Reports

Author:

Bettencourt Jacob W.,McLaury Alex R.,Limberg Afton K.,Vargas-Hernandez Juan S.,Bayram Banu,Owen Aaron R.,Berry Daniel J.,Sanchez-Sotelo Joaquin,Morrey Mark E.,van Wijnen Andre J.^ORCID,Abdel Matthew P.^ORCID

Funder

National Institutes of Health

Anna-Maria and Stephen Kellen Foundation

Publisher

Elsevier BV

Subject

Genetics

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3. Beta-actin as a loading control: less than 2 mug of total protein should be loaded;Chen;Electrophoresis,2015

4. Beta-actin is not a reliable loading control in Western blot analysis;Dittmer;Electrophoresis,2006

5. Total protein analysis as a reliable loading control for quantitative fluorescent Western blotting;Eaton;PLoS One,2013

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