Roseomonas riguiloci sp. nov., isolated from wetland freshwater

Abstract

A non-motile, coccobacillus-shaped and pink pigmented bacterium, designated strain 03SU10-PT, was isolated from wetland freshwater (Woopo wetland, Republic of Korea). Cells were Gram reaction-negative and catalase- and oxidase-positive. The major fatty acids (>10 % of total) were C18 : 1ω7cand summed feature 3 (iso-C15 : 02-OH and/or C16 : 1ω7c). The predominant respiratory lipoquinone was Q-10. The DNA G+C content was 68 mol%. The major polar lipids were phosphatidylethanolamine, phosphatidylcholine and an unknown aminolipid. Spermidine, putrescine and 1,3-diaminopropane were the major polyamines. A phylogenetic tree based on 16S rRNA gene sequence comparisons showed that strain 03SU10-PTformed an evolutionary lineage within the radiation enclosing the members of the genusRoseomonas. The nearest neighbour to the novel strain wasRoseomonas stagniHS-69T(96.3 % gene sequence similarity). The evidence provided by the polyphasic taxonomic approach used in this study indicated that strain 03SU10-PTcould not be assigned to any recognized species; therefore a novel species is proposed,Roseomonas riguilocisp. nov., with 03SU10-PT( = KCTC 23339T = JCM 17520T) as the type strain.