Affiliation:
1. Department of Zoology, University of Delhi, Delhi-110007, India
2. Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7B, D-38124 Braunschweig, Germany
Abstract
A Gram-staining-positive, heterotrophic, aerobic, non-motile, non-endospore-forming, yellow-coloured rod, designated strain N5T, was isolated from a soil sample collected at an industrial waste site in Noida, on the outskirts of Delhi, India. In phylogenetic analyses based on 16S rRNA gene sequences, strain N5T was most closely related to members of established species in the genus
Microbacterium
(with sequence similarities of approximately 94.0–97.6 %), particularly
Microbacterium indicum
LMG 23459T (97.59 %) and
Microbacterium gubbeenense
LMG 19263T (97.18 %). In DNA–DNA hybridization studies, however, none of the DNA–DNA relatedness values between strain N5T and members of the genus
Microbacterium
exceeded 11.3 %. The genomic DNA G+C content of the novel strain was 68 mol%. The chemotaxonomic characteristics of strain N5T, which had MK-11 and MK-10 as its major menaquinones and anteiso-C15 : 0 (45 %), anteiso-C17 : 0 (37 %), iso-C16 : 0 (8.5 %) and C16 : 0 (4.5 %) as its predominant fatty acids, were consistent with classification in the genus
Microbacterium
. Peptidoglycan in the novel strain, which contained ornithine, alanine, glycine, homoserine, glutamic acid, 3-hydroxyglutamic acid, muramic acid and traces of N-glycolyl residues, was of type B2β. The polar lipid profile of strain N5T comprised diphosphatidylglycerol, phosphatidylglycerol and an unknown glycolipid. The novel strain’s major cell-wall sugars were glucose and galactose. Based on the phylogenetic, DNA–DNA hybridization, chemotaxonomic and phenotypic data, strain N5T represents a novel species within the genus
Microbacterium
for which the name Microbacterium amylolyticum sp. nov. is proposed; the type strain is N5T ( = DSM 24221T = CCM 7881T).
Funder
Government of India’s Council of Scientific and Industrial Research and Department of Biotechnology
Promotion of University Research and Scientific Excellence grant
Indian National Bureau of Agriculturally Important Micro-organisms
Government of India’s Department of Biotechnology
University of Delhi’s Department of Science and Technology
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology
Cited by
23 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献