Brevibacillus ginsengisoli sp. nov., a denitrifying bacterium isolated from soil of a ginseng field

Abstract

A Gram-positive, rod-shaped, spore-forming bacterium, Gsoil 3088T, was isolated from soil from a ginseng field in Pocheon Province in South Korea and characterized in order to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarity, strain Gsoil 3088Twas shown to belong to the familyPaenibacillaceae, being related toBrevibacillus centrosporus(96.6 %),Brevibacillus borstelensis(96.3 %),Brevibacillus parabrevis(96.1 %),Brevibacillus formosus(96.1 %),Brevibacillus brevis(96.1 %) andBrevibacillus laterosporus(96.0 %). The phylogenetic distances from other validly described species within the genusBrevibacilluswere greater than 4.0 % (i.e. there was less than 96.0 % similarity). The G+C content of the genomic DNA was 52.1 mol%. Phenotypic and chemotaxonomic data (major menaquinone, MK-7; fatty acid profile, iso-C15 : 0, iso-C14 : 0and anteiso-C15 : 0) supported the affiliation of strain Gsoil 3088Tto the genusBrevibacillus. The results of physiological and biochemical tests allowed strain Gsoil 3088Tto be distinguished genotypically and phenotypically fromBrevibacillusspecies with validly published names. Strain Gsoil 3088T, therefore, represents a novel species of the genusBrevibacillus, for which the nameBrevibacillus ginsengisolisp. nov. is proposed. The type strain is Gsoil 3088T(=KCTC 13938T=LMG 23403T).