Elizabethkingia endophytica sp. nov., isolated from Zea mays and emended description of Elizabethkingia anophelis Kämpfer et al. 2011

Abstract

A slightly yellow bacterial strain (JM-87T), isolated from the stem of healthy 10 day-old sweet corn (Zea mays), was studied for its taxonomic allocation. The isolate revealed Gram-stain-negative, rod-shaped cells. A comparison of the 16S rRNA gene sequence of the isolate showed 99.1, 97.8, and 97.4 % similarity to the 16S rRNA gene sequences of the type strains of Elizabethkingia anophelis, Elizabethkingia meningoseptica and Elizabethkingia miricola, respectively. The fatty acid profile of strain JM-87T consisted mainly of the major fatty acids C15:0 iso, C17:0 iso 3-OH, and C15:0 iso 2-OH/C16:1ω7c/t. The quinone system of strain JM-87T contained, exclusively, menaquinone MK-6. The major polyamine was sym-homospermidine. The polar lipid profile consisted of the major lipid phosphatidylethanolamine plus several unidentified aminolipids and other unidentified lipids. DNA–DNA hybridization experiments with E. meningoseptica CCUG 214T ( = ATCC 13253T), E. miricola KCTC 12492T ( = GTC 862T) and E. anophelis R26T resulted in relatedness values of 17 % (reciprocal 16 %), 30 % (reciprocal 19 %), and 51 % (reciprocal 54 %), respectively. These DNA–DNA hybridization results, in addition to some differentiating biochemical properties, clearly indicate that strain JM-87T is a representative of a novel species, for which the name Elizabethkingia endophytica sp. nov. is proposed. The type strain is JM-87T ( = CIP 110885T = LMG 28604T = CCM 8570T).