Affiliation:
1. State Key Laboratory for Molecular Virology and Genetic Engineering, Institute of Virology, Chinese Academy of Preventive Medicine, 100 Yingxin Street, Xuanwu District, Beijing 100052, People’s Republic of China1
Abstract
Infection with alphaviruses is common in the Chinese population. Here we report the isolation of a Sindbis-like virus from a pool of Anopheles mosquitoes collected in Xinjiang, China during an arbovirus survey. This virus, designated XJ-160, rapidly produced cytopathic effects on mosquito and hamster cells. In addition, it was lethal to neonatal mice if inoculated intracerebrally. Serologically, XJ-160 reacted with and was neutralized by an anti-Sindbis antibody. Anti-XJ-160 antibodies were found in several cohorts of Chinese subjects. The complete 11626-base nucleotide sequence of XJ-160 was determined. XJ-160 has diverged significantly from the prototype Sindbis virus, with an 18% difference in nucleotide sequence and an 8·6% difference in amino acids; there are 11 deletions and 2 insertions, involving 99 nucleotides in total. XJ-160 is most closely linked to Kyzylagach virus isolated in Azerbaijan. Both belong to the African/European genetic lineage of Sindbis virus, albeit more distantly related to other members.
Reference23 articles.
1. Arbovirus survey in China in recent ten years;Chen;Chinese Medical Journal (English),1996
2. Arbovirus antibody survey of human sera in China;Chen;Chinese Journal of Epidemiology,1983
3. A single amino acid change in the E2 glycoprotein of Sindbis virus confers neurovirulence by altering an early step of virus replication;Dropulic;Journal of Virology,1997
4. Genetic determinants of Sindbis virus neuroinvasiveness;Dubuisson;Journal of Virology,1997
5. Survey of arbovirus in China;Liang;Chinese Journal of Zoonoses,1997
Cited by
33 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献