Affiliation:
1. College of Grassland Science, Xinjiang Agricultural University, Urumqi 830052, PR China
2. Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences, Xinjiang Laboratory of Special Environmental Microbiology, Urumqi, Xinjiang 830091, PR China
3. College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, PR China
Abstract
A Gram-negative, rod-shaped, aerobic and light pink-pigmented bacterium, designated XAAS-A31T, was isolated from the soil of a Populus euphratica forest located near Hotan City, Xinjiang, PR China. Polyphasic, taxonomic and phylogenomic analyses were used to determine the taxonomy position of the strain. Phylogenetic analysis based on 16S rRNA gene sequence analysis indicated that XAAS-A31T belongs to the genus
Pontibacter
, family
Hymenobacteraceae
, and shows highest sequence similarity to
Pontibacter silvestris
XAAS-R86T (96.2 %). The digital DNA–DNA hybridization (22.0 %–19.2 %) and orthologous average nucleotide identity (74.1 %–72.7 %) values relative to closest validly published
Pontibacter
species were lower than the recommended thresholds of 70 and 96 %, respectively. The cells grew at 4–40 °C (optimum, 28–30 °C), at pH 6.5–8.5 (pH 7.0–7.5) and with 0–8% NaCl (0.5–2.0% NaCl). The main respiratory quinone of XAAS-A31T is MK-7, and the principal cellular fatty acids are iso-C15 : 0, iso-C17 : 0 3OH and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B). The major polar lipids are phosphatidylethanolamine, three unidentified amino-phospholipids, one unidentified glycolipid and eight unidentified lipids. The genome length of strain XAAS-A31T is 5.48 Mbp with a DNA G+C content of 44.2 mol% and 4013 protein-coding genes. Phenotypic and genotypic data suggested that XAAS-A31T represents a novel
Pontibacter
species, for which we propose the name Pontibacter harenae sp. nov. and type strain XAAS-A31T (=CCTCC AB 2017162T=KCTC 62049T).
Funder
Project of Renovation Capacity Building for the Young Sci-Tech Talents Sponsored by Xinjiang Academy of Agricultural Sciences
Subject
General Medicine,Ecology, Evolution, Behavior and Systematics,Microbiology