Abstract
In this chapter, we present mixed-mode stationary phases and their applications in the determination of nonpolar, polar, and charged compounds, as well as larger molecules such as peptides or proteins using a single column. Mixed-mode chromatography (MMC) has been growing rapidly in recent years, owing to the new generation of mixed-mode stationary phases and a better understanding of multimode interactions. Mixed-mode chromatography provides a wide range of selectivities and adequate retention of a variety of compounds, especially polar and charged molecules. In summary, this technique is particularly useful in the pharmaceutical analysis of drugs, impurities, biopharmaceuticals, and polar compounds in natural products.