Author:
Rencuzogullari Ozge,Ozfiliz-Kilbas Pelin,Bal Enes,Ayhan-Sahin Burcu
Abstract
The tumor microenvironment increases the growth and invasion of cancer cells, makes classical chemotherapy applications inadequate, and is associated with a poor cancer prognosis. Recent studies reveal that cancer stroma supports tumor growth and metastasis and develops resistance to chemotherapy. In vitro co-culture techniques are widely used to study cross-talk between tumor microenvironment cells such as adipocytes, endothelial cells, fibroblasts, macrophages, and cancer cells. Co-culture techniques are classified into two main categories: indirect and direct methods. Transwell (indirect) co-culture of mature adipocytes with cancer cells has shown cancer cell viability, growth, proliferation, invasion, and metastases. This chapter covers the general methods of co-culture studies and will emphasize the results obtained on the co-culture of adipocytes and cancer cells.
Reference96 articles.
1. Kontomanolis EN, Koutras A, Syllaios A, et al. Basic principles of molecular biology of cancer cell-molecular cancer indicators. Journal of Balkan Union of Oncology. 2021;26:1723-1734
2. Vaghari-Tabari M, Ferns GA, Qujeq D, et al. Signaling, metabolism, and cancer: An important relationship for therapeutic intervention. Journal of Cellular Physiology. 2021;236:5512-5532
3. Zaimy MA, Saffarzadeh N, Mohammadi A, et al. New methods in cancer diagnosis and gene therapy of cancer-based on nanoparticles. Cancer Gene Therapy. 2017;24:233-243
4. Song Q , Merajver SD, Li JZ. Cancer classification in the genomic era: Five contemporary problems. Human Genomics. 2015;9:27
5. Mendiratta G, Ke E, Aziz M, et al. Cancer gene mutation frequencies for the U.S. population. Nature Communications. 2021;12:5961