Author:
Armstrong D. T.,Kotaras P. J.,Earl C. R.
Abstract
The use of juvenile donors in embryo-transfer (ET) programmes offers
considerable potential for accelerated genetic gain in domestic livestock
through reduced generation interval. The present paper reviews recent research
aimed at optimizing embryo production from oocytes collected from young calves
and lambs using in vitro methods of embryo production.
Emphasis is placed on criteria for donor selection, oocyte-collection methods,
and hormone-stimulation methods designed to produce maximum yields of viable
oocytes. In vitro fertilization (IVF) rates of calf and
lamb oocytes did not differ significantly whether matured
in vivo or in vitro, and rates of
development to blastocyst stages in culture were similar to those observed for
embryos derived from adult donors. Blastocysts produced by IVF of lamb and
calf oocytes established ET pregnancies at rates of 30–45%.
Pregnant recipients have reached full term and delivered normal offspring at
rates similar to those expected following ET of embryos produced
in vivo from superovulated donors. On the basis of
current follicle-stimulation protocols, on rates of blastocyst production in
vitro under optimal conditions, and on observed pregnancy rates from fresh
transfer of IVF embryos, 8–10 pregnancies may be expected per oocyte
collection from 10–12-week-old calves and from 6–8-week-old lambs.
Subject
Developmental Biology,Endocrinology,Genetics,Molecular Biology,Animal Science and Zoology,Reproductive Medicine,Biotechnology
Cited by
82 articles.
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