Influence of Adsorbent Nature on the Dynamic Headspace Study of Insect Semiochemicals

Abstract

In chemical ecology studies (insect–insect, insect–plant relationships), it is important to choose the appropriate sampling methods and the correct optimization of sampling by using dynamic systems. In the present work, different adsorbents were evaluated in a dynamic system that presents a stream of purified air flowing through an aeration chamber containing a mixture of volatile organic compounds, mainly insect semiochemicals such as α-pinene, sulcatone, β-linalool, menthone, isomenthone, methyl salicylate, grandlure I, grandlure II, grandlure III, grandlure IV, eugenol, and α-ionone. Traditional adsorbents such as Tenax TA, Porapak Q, Hayesep Q, and activated charcoal were evaluated; further, alternatives such as Porapak Rxn RP, HLB, SCX, and silica gel, among others were proposed owing to their lower cost. The results demonstrated that Porapak Q and Porapak Rxn RP, despite their different chemical composition, were able to produce similar ratios of compounds to that of the reference solution and, moreover, with the highest recovery yields. However, it is important to emphasize the adsorption selectivity provided by SCX for eugenol and α-ionone. When Porapak Rxn RP was used in the analysis of Eucalyptus globulus volatiles, excellent results were obtained, and these agree with reported data from a hydrodistillation method.