Effect of voluntary wheel running on autophagy status in lung tissue of high-fat diet-fed rats-Reference-Cited by-同舟云学术

Effect of voluntary wheel running on autophagy status in lung tissue of high-fat diet-fed rats

Published:2023-04-03 Issue:2 Volume:19 Page:175-181
ISSN:1755-2540
Container-title:Comparative Exercise Physiology
language:
Short-container-title:CEP

Author:

Rezaie J.¹,Aboulhassani A.²³,Keyhanmanesh R.⁴,Rahbarghazi R.⁵⁶,Delkhosh A.⁵,Salimi L.⁵,Zamani A.R. Nezhad⁵,Rahbarghazi A.⁷,Ahmadi M.⁵,Ghiasi F.⁸

Affiliation:

1. Solid Tumor Research Center, Cellular and Molecular Medicine Institute, Urmia University of Medical Sciences, 5714783734 Urmia, Iran.

2. Student Research Committee, Tabriz University of Medical Sciences, 5165665931 Tabriz, Iran.

3. Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, 5165665931 Tabriz, Iran.

4. Drug Applied Research Center, Tabriz University of Medical Sciences, 5165665931 Tabriz, Iran.

5. Stem Cell Research Center, Tabriz University of Medical Sciences, 5165665931 Tabriz, Iran.

6. Department of Applied Cell Sciences, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, 5165665931 Tabriz, Iran.

7. Department of Physical Education and Sports Sciences, Faculty of Educational Science and Psychology, University of Mohaghegh Ardabil, 56199-11367 Ardabil, Iran.

8. Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Abstract

Here, we aimed to explore the therapeutic effect of voluntary wheel running (VWR) in high-fat diet-fed rats on pulmonary tissue injury via the modulation of autophagic response. Thirty-two rats were allocated into four groups; normal diet (Control); VWR; high-fat-diet (HFD), and HFD + VWR. After three months, pathological effect of HFD on pulmonary tissue was investigated. The levels of tumour necrosis factor (TNF)-α were detected in the bronchoalveolar lavage fluid (BALF). We monitored the expression of interleukin (IL)-6 and autophagy-related genes in lung tissues. H&E staining showed pathological changes in HFD group coincided with the increase of TNF-α levels in the bronchoalveolar fluid compared to the normal rats. Our results showed the up-regulation of IL-6, becline-1, LC3 and P62 in the HFD group compared to the Control group. VWR inhibited HFD-induced changes and could decrease HFD-induced changes via the regulation of autophagy status.

Publisher

Brill

Subject

Physiology (medical),Veterinary (miscellaneous),Orthopedics and Sports Medicine,Physiology,Biochemistry,Endocrinology, Diabetes and Metabolism,Biophysics

Reference30 articles.

1. Monitoring IL-13 expression in relation to miRNA-155 and miRNA-133 changes following intra-tracheal administration of mesenchymal stem cells and conditioned media in ovalbuminsensitized rats

2. Effect of high fat diet on NF-кB microRNA146a negative feedback loop in ovalbumin-sensitized rats

3. Modification of lung endoplasmic reticulum genes expression and NF-kB protein levels in obese ovalbumin-sensitized male and female rats

4. Bayazidi, M.G., Rahbarghazi, R., Rezabakhsh, A., Rezaie, J., Hassanpour, M. and Ahmadi, M., 2022. Type 2 diabetes mellitus induced autophagic response within pulmonary tissue in the rat model. BioImpacts. https://doi.org/10.34172/bi.2022.22183

5. High-Fat-Diet-Induced Obesity and Heart Dysfunction Are Regulated by the TOR Pathway in Drosophila