Characterization and antibacterial activity of alkaloids and polyphenols extracts from Haplophyllum tuberculatum (Forssk.) A. Juss.
Author:
DJOUAHRA FAHEM Djamila1ORCID, BENSMAIL Souhila1ORCID, BOUTELDJA Razika1ORCID, SARA Messad1ORCID, FERHOUM Fatiha1ORCID, BOURFIS Nassima1ORCID, ACHEUK Fatma2ORCID, FAZOUANE Fethia2ORCID
Affiliation:
1. Akli Mohand Oulhadj 2. M'Hamad Bougara
Abstract
Haplophyllum tuberculatum is a medicinal plant belonging to the Rutaceae family, is renowned for its various therapeutic properties. This study aims to characterize the alkaloids and polyphenol extracts from this plant and assess their antibacterial activity. Herein, the extraction of polyphenols and alkaloids from this plant was performed by the maceration method. Folin Ciacalteu’s method was used to estimate the total phenolic content, and the qualitative characterization of the two extracts was performed by thin-layer chromatography. Whilst, the antibacterial activity of the two extracts was tested with the disk diffusion method on a solid medium and the minimal inhibitory concentration (MIC) of susceptible bacteria was determined using the agar dilution method. Our results indicate respective yields of 8.39% in polyphenols and 0.37% in alkaloid extracts, while the total phenolic content was estimated to be 74.45 mg GAE/g of dry matter in polyphenolic extract. Thin-layer chromatography analysis allowed choosing the system toluene-acetate-ethanol-concentrated ammonia (40:4:8:3, v/v) to separate H. tuberculatum alkaloids, and ethyl acetate-methanol-water (100:13.5:10, v/v/) for the separation of its polyphenols. The same analysis detected traces of quercitin, catechin, and rutin in the polyphenolic extract. Our findings demonstrated good antibacterial activity on Gram-positive strains such as Staphylococcus aureus ATCC 25923 and Bacillus subtilis ATCC 6633, and moderate activity on Pseudomonas aeruginosa ATCC 27953, with MICs ranging from 0.625 to 10 mg/mL for alkaloids and from 5 to 20 mg/mL for polyphenols.
Publisher
International Journal of Secondary Metabolite
Subject
Plant Science,Biochemistry,Biophysics,Biotechnology
Reference46 articles.
1. Abdelgaleil, S., Saad, M., Ariefta, N., & Shiono, Y. (2020). Antimicrobial and phytotoxic activities of secondary metabolites from Haplophyllum tuberculatum and Chrysanthemum coronarium. South African Journal of Botany, 128, 35 41. https://doi.org/10.1016/j.sajb.2019.10.005 2. Abdelkhalek, A., Salem, M.Z., Hafez, E., Behiry, S.I., & Qari, S.H. (2020). The phytochemical, antifungal, and first report of the antiviral properties of Egyptian Haplophyllum tuberculatum extract. Biology, 9(9), 248. https://doi.org/10.3390/biology9090248 3. Aboshora, W., Lianfu, Z., Dahir, M., Qingran, M., Qingrui, S., Jing, L., Al-Haj, N.Q.M., & Ammar, A. (2014). Effect of extraction method and solvent power on polyphenol and flavonoid levels in Hyphaene thebaica L mart (Arecaceae) (Doum) fruit, and its antioxidant and antibacterial activities. Tropical Journal of Pharmaceutical Research, 13(12), 2057-2063. https://doi.org/10.4314/tjpr.v13i12.16 4. Abou-Zeid, H.M., Bidak, L.M., & Gohar, Y.M. (2014). Phytochemical screening and antimicrobial activities of some wild medicinal plants of the western mediterranean coastal region, Egypt. International Journal of Pharmaceutical Sciences and Research 5(7), 3072-3080. http://doi.org/10.13040/IJPSR.0975-8232.5(10).4445-55 5. Al-Brashdi, A.S., Al-Ariymi, H., Al Hashmi, M., & Khan, S.A. (2016). Evaluation of antioxidant potential, total phenolic content and phytochemical screening of aerial parts of a folkloric medicine, Haplophyllum tuberculatum (Forssk) A. Juss. Juss Journal of Coastal Life Medicine, 4, 315-319. https://doi.org/10.12980/jclm.4.2016J6-4
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