Author:
NAIDU J SHARANYA,ARANGASAMY A,BACKIALAKSHMI S,SELVARAJU S,REDDY I J,BHATTA R
Abstract
This study aimed to investigate the influence of calcium and magnesium supplementation on the skewing of sex ratio and placental genes expression in New Zealand White Rabbits. A total of 25 rabbits were allocated to 5 groups; each treatment group was supplemented with Ca and Mg; T1 (0.4% and 0.01%), T2 (0.6% and 0.02%), T3 (0.8% and 0.03%), and T4 (1.0% and 0.04%) respectively, while the C group was provided with regular feed and subjected to three breeding. The T3 group produced a female-biased litter and hence, the F1 female kits (n=12) of T3 and C group was provided same supplementation management. At 21 days of gestation, three animals from each group were sacrificed and placental samples were collected, the remaining animals were allowed for full-term delivery. The selected F1 produced female-biased litter with elevated serum Ca and Mg concentrations and reduced sodium and cholesterol levels. A total of 15 genes related to mineral absorption, placental development and immunity were selected to study the influence of diet on sex and placental gene expression. The expression of genes such as PEG10, SOD1, SLC30A, TLR4, AR, and TRPM6 was high in the treatment placenta compared to the control. RTL1, ESR2, CALM2, and TRPM6 upregulated in the treatment female placenta. The study concluded that the intake of 0.8% Ca and 0.03% Mg could lead to the production of more females and upregulation of some placental genes could serve as the molecular mechanism in response to intake of the minerals and fetal sex.
Publisher
Indian Council of Agricultural Research, Directorate of Knowledge Management in Agriculture
Subject
General Veterinary,Animal Science and Zoology
Reference33 articles.
1. Abd Elraouf Oun S B, Soltan S, Taha A and Kadry E. 2016. Preconceptional minerals administration skewed sex ratio in rat offspring. Research Journal of Obstetrics and Gynecology 7: 11–15.
2. Alhimaidi A R, Ammari A A, Alghadi M Q, al Saiady M Y, Amran R A and Swelum A A. 2021. Sex preselection of sٍheep embryo by altering the minerals of maternal nutrition. Saudi Journal of Biological Sciences 28(1): 680–4.
3. Arangasamy A, Selvaraju S, Parthipan S, Somashekar L, Rajendran D and Ravindra J P. 2015. Role of calcium and magnesium administration on sex ratio skewing, follicular fluid protein profiles and steroid hormone level and oocyte transcripts expression pattern in Wistar rat. Indian Journal of Animal Sciences 85(11): 1190–4.
4. Cameron E Z, Lemons P R, Bateman P W and Bennett N C. 2008. Experimental alteration of litter sex ratios in a mammal. Proceedings of the Royal Society B: Biological Sciences 275(1632): 323–7.
5. Chow S N, Chien C H, Chen Y P and Ouyang P C. 1987. Purification and characterization of metal-binding proteins and the corresponding mRNA from human placentas. Biological Research in Pregnancy and Perinatology 8(2 2D Half): 65–72.