In vitro propagation of Gypsophila germanicopolitana Hub.-Mor. an endangered and edaphic endemic in Çankırı

Abstract

In this study, in vitro propagation of Gypsophila germanicopolitana HUB.- MOR., which grows on gypsum hills within the limits of Çankırı province was taken as an aim. The species is included in the list of plants under “critical extinction (CR)” according to the International Union for Conservation of Nature and Natural Resources red list category. Shoot tips and internodes of the plant were used as explant sources for in vitro propagation, as basal nutrient medium 1) Murashige and Skoog (MS) 2) Nitsch & Nitsch (NN) were chosen, as plant growth regulators 1) 6-benzylaminopurine (BAP) (0 mg/L, 0.5 mg/L, 1 mg/L) and 2) Kinetin (KIN) (0 mg/L, 0.5 mg/L, 1 mg/L) were used as cytokinin source as well as 3) Indole-3-butyric acid (IBA) (0 mg/L) L, 0.25 mg/L, 0.5 mg/L) and 4) α-naphthalene acetic acid (NAA) (0 mg/L, 0.25 mg/L, 0.5 mg/L) were as auxin sources. After that 36 combinations with different doses were established. Gelrite (2.1 g/L) was used as a gelling agent and 30 g/L of sucrose was added as a carbon source to the nutrient media. As a result, the best shoot regeneration was observed in Nitsch & Nitsch (NN) nutrient medium with a combination of 0 mg/L KIN + 0.5 mg/L NAA plant growth regulator. Root regeneration was more successfully present within the explants cultured in MS nutrient medium than in NN nutrient medium. No other in vitro propagation studies have been carried out on this endemic species before and so it has a unique value in serving as a basis for future studies.