Effects of preconditioning with heat stress on acute exercise‐induced intracellular signaling in male rat gastrocnemius muscle

Author:

Yoshihara Toshinori1ORCID,Dobashi Shohei12,Naito Hisashi1

Affiliation:

1. Graduate School of Health and Sports Science Juntendo University Chiba Japan

2. Institute of Health and Sport Sciences University of Tsukuba Ibaraki Japan

Abstract

AbstractHeat stress (HS) induces Akt/mTOR phosphorylation and FoxO3a signaling; however, whether a prior increase in heat shock protein 72 (HSP72) expression affects intracellular signaling following eccentric exercise remains unclear. We analyzed the effects of HS pretreatment on intramuscular signaling in response to acute exercise in 10‐week‐old male Wistar rats (n = 24). One leg of each rat was exposed to HS and the other served as an internal control (CT). Post‐HS, rats were either rested or subjected to downhill treadmill running. Intramuscular signaling responses in the red and white regions of the gastrocnemius muscle were analyzed before, immediately after, or 1 h after exercise (n = 8/group). HS significantly increased HSP72 levels in both deep red and superficial white regions. Although HS did not affect exercise‐induced mTOR signaling (S6K1/ERK) responses in the red region, mTOR phosphorylation in the white region was significantly higher in CT legs than in HS legs after exercise. Thr308 phosphorylation of Akt showed region‐specific alteration with a decrease in the red region and an increase in the white region immediately after downhill running. Overall, a prior increase in HSP72 expression elicits fiber type‐specific changes in exercise‐induced Akt and mTOR phosphorylation in rat gastrocnemius muscle.

Funder

Japan Society for the Promotion of Science

Publisher

Wiley

Subject

Physiology (medical),Physiology

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