Apoptosis-related gene expression induced by Colombian propolis samples in canine osteosarcoma cell line

Author:

Pardo-Mora Dolly Patricia1ORCID,Murillo Oscar Julián2ORCID,Rey-Buitrago Mauricio3ORCID,Losada-Barragán Mónica1ORCID,Uribe Jaime Fabian Cruz1ORCID,Santiago Karina Basso4ORCID,Conti Bruno José4ORCID,Cardoso Eliza de Oliveira4,Conte Fernanda Lopes4ORCID,Gutiérrez Rafael María1ORCID,García Orlando Torres1ORCID,Sforcin José Maurício4ORCID

Affiliation:

1. Department of Animal Health, Facultad de Medicina Veterinaria, Universidad Antonio Nariño, Bogotá, Colombia.

2. Department of Morphology, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá, Colombia.

3. Department of Animal Health, Facultad de Medicina Veterinaria, Universidad Antonio Nariño, Bogotá, Colombia; Department of Morphology, Facultad de Medicina, Universidad Nacional de Colombia, Bogotá, Colombia.

4. Department of Chemical and Biological Sciences, São Paulo State University (UNESP), Institute of Biosciences, Campus Botucatu, Brazil.

Abstract

Background and Aim: Osteosarcoma (OSA) is the most common bone tumor in canines and humans. This study aimed to assess the cytotoxic and apoptotic effects of Colombian propolis samples on a canine OSA cell line (OSCA-8) by evaluating the expression of BCL-2, BAX, CASPASE 9, CASPASE 8, and TNFR1 genes involved in the apoptosis pathway. Materials and Methods: After treating the cells with five Colombian propolis samples (Usm, Met, Fus, Sil, and Caj), we evaluated cell viability and lactate dehydrogenase (LDH) release. Early and late apoptosis was determined by flow cytometry using annexin V/propidium iodide. Furthermore, the effects of three selected samples on gene expression were analyzed by real-time polymerase chain reaction. Results: The Colombian propolis samples reduced OSCA-8 cell viability and increased LDH release. All samples induced apoptosis significantly and upregulated BCL-2 and CASPASE 8 expression. Usm and Sil increased BAX expression, Met and Sil induced CASPASE 9 expression, and Usm increased TNFR1. Conclusion: Colombian propolis samples exhibited cytotoxic and apoptotic effects on canine OSA cells, and CASPASE 8 upregulation indicated apoptosis induction by the extrinsic pathway.

Funder

Universidad Autónoma Agraria Antonio Narro

Publisher

Veterinary World

Subject

General Veterinary

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