Investigation of methicillin-resistant Staphylococcus aureus, methicillin-susceptible Staphylococcus aureus, and Staphylococcus argenteus from wild long-tailed macaques (Macaca fascicularis) at Kosumpee Forest Park, Maha Sarakham, Thailand
-
Published:2022-11-26
Issue:
Volume:
Page:2693-2698
-
ISSN:2231-0916
-
Container-title:Veterinary World
-
language:en
-
Short-container-title:Vet World
Author:
Pumipuntu Natapol1ORCID, Chamnandee Thanyaphorn2, Saengthong Kittisak2, Pathomthanasarn Suvit2, Tanee Tawatchai3ORCID, Kyes Pensri4ORCID, Thamsenanupap Penkhae3ORCID, Karaket Apichat5, Roberts Marilyn C.6ORCID, Kyes Randall C.7ORCID
Affiliation:
1. One Health Research Unit, Mahasarakham University, Maha Sarakham, Thailand; Veterinary Infectious Disease Research Unit, Mahasarakham University, Maha Sarakham, Thailand; Faculty of Veterinary Sciences, Mahasarakham University, Maha Sarakham, Thailand. 2. One Health Research Unit, Mahasarakham University, Maha Sarakham, Thailand; Faculty of Veterinary Sciences, Mahasarakham University, Maha Sarakham, Thailand. 3. One Health Research Unit, Mahasarakham University, Maha Sarakham, Thailand; Faculty of Environment and Resource Studies, Mahasarakham University, Maha Sarakham, Thailand. 4. Department of Psychology, Center for Global Field Study, and Washington National Primate Research Center, University of Washington, Seattle, Washington, USA. 5. Department of National Parks, Wildlife and Plant Conservation, Bangkok, Thailand. 6. Department of Environmental and Occupational Health, University of Washington, Seattle, Washington, USA. 7. Departments of Psychology, Global Health, and Anthropology, Center for Global Field Study, and Washington National Primate Research Center, University of Washington, Seattle, Washington, USA.
Abstract
Background and Aim: In the past, the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) infections in both humans and animals has increased across Thailand. Staphylococcus argenteus has been associated with infections among humans, exotic pets, and livestock. Both species have been identified in non-human primate species from geographically diverse locations but not from non-human primates in Thailand. This study aimed to determine the presence of MRSA/ methicillin-susceptible S. aureus (MSSA) and S. argenteus isolates collected from buccal swab samples in Macaca fascicularis at Kosumpee Forest Park (KFP), Maha Sarakham, Northeast Thailand.
Materials and Methods: Aseptic buccal swab samples were collected from 30 free-ranging macaques in November 2018. All isolates were tested using multiple biochemical tests and S. aureus latex slide agglutination test. Presumptive S. aureus isolates were tested for the presence of the mecA gene using polymerase chain reaction (PCR) assays. The isolates were phenotypically determined to be resistant to a β-lactam antibiotic using the disk diffusion method with a 30 μg cefoxitin disk. The isolates were analyzed by PCR for the non-ribosomal peptide synthetase (NRPS) gene to distinguish S. argenteus from S. aureus.
Results: Fifteen macaques (50%) were colonized with S. aureus and 21 isolates were characterized. Three of the macaques carried both the MRSA and MSSA isolate. One animal carried both MRSA and S. argenteus isolate, and one animal carried only S. argenteus. The NRPS gene analysis confirmed that 2 isolates (9.52%) were S. argenteus and 19 isolates (90.48%) were S. aureus [five MSSA and 14 MRSA].
Conclusion: This study is the first to identify MRSA/MSSA and S. argenteus in wild free-ranging M. fascicularis from Thailand at the KFP in Maha Sarakham. This study is also the first report on the occurrence of S. argenteus carriage in M. fascicularis from Thailand.
Funder
Mahasarakham University Office of Research Infrastructure Programs, National Institutes of Health
Publisher
Veterinary World
Subject
General Veterinary
Reference32 articles.
1. Malaivijitnond, S. and Hamada, Y. (2008) Current situation and status of long tailed macaques (Macaca fascicularis) in Thailand. Nat. Hist. J. Chulalongkorn Univ., 8(2): 185–204. 2. Roberts, M.C., Joshi, P.R., Monecke, S., Ehricht, R., Müller, E., Gawlik, D., Paudel, S., Acharya, M., Bhattarai, S., Pokharel, S., Tuladhar, R., Chalise, M.K. and Kyes, R.C. (2019) MRSA strains in Nepalese rhesus macaques (Macaca mulatta) and their environment. Front. Microbiol., 10 : 2505. 3. Roberts, M.C., Joshi, P.R., Monecke, S., Ehricht, R., Müller, E., Gawlik, D., Diezel, C., Braun, S.D., Paudel, S., Acharya, M., Khanal, L., Koju, N.P., Chalise, M. and Kyes, R.C. (2020) Staphylococcus aureus and methicillin-resistant S. aureus in Nepalese primates: Resistance to antimicrobials, virulence, and genetic lineages. Antibiotics, 9(10): 689. 4. Schurer, J.M., Ramirez, V., Kyes, P., Tanee, T., Patarapadungkit, N., Thamsenanupap, P., Trufan, S., Grant, E.T., Garland-Lewis, G., Kelley, S., Nueaitong, H., Kyes, R.C. and Rabinowitz, P. (2019) Long-tailed macaques (Macaca fascicularis) in urban landscapes: Gastrointestinal parasitism and barriers for healthy coexistence in northeast Thailand. Am. J. Trop. Med. Hyg., 100(2): 357–364. 5. Damrongsukij, P., Doemlim, P., Kusolsongkhrokul, R., Tanee, T., Petcharat, P., Siriporn, B., Piratae, S. and Pumipuntu, N. (2021) One health approach of melioidosis and gastrointestinal parasitic infections from Macaca fascicularis to human at Kosumpee forest park, Maha Sarakham, Thailand. Infect. Drug Resist., 15(14): 2213–2223.
Cited by
5 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献
|
|