Realistic aspects behind the application of the rat model of chemically-induced mammary cancer: Practical guidelines to obtain the best results
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Published:2023-06
Issue:
Volume:
Page:1222-1230
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ISSN:2231-0916
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Container-title:Veterinary World
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language:en
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Short-container-title:Vet World
Author:
Silva Jéssica1ORCID, Duarte José Alberto2ORCID, Oliveira Paula A.3ORCID
Affiliation:
1. Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Vila Real, Portugal; Institute for Innovation, Capacity Building and Sustainability of Agri-food Production (Inov4Agro), Vila Real, Portugal. 2. Research Center for Physical Activity, Health and Leisure (CIAFEL), Faculty of Sport, University of Porto, Porto, Portugal; Toxicology Research Unit (TOXRUN), Advanced Polytechnic and University Cooperative (CESPU), Gandra, Portugal. 3. Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Vila Real, Portugal; Institute for Innovation, Capacity Building and Sustainability of Agri-food Production (Inov4Agro), Vila Real, Portugal; Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal.
Abstract
Cancer is one of the most important public health problems worldwide. Despite the great contribution of in-vitro studies for biomedical research, animals are essential to study diseases’ biopathology and diagnosis, and searching for new preventive and therapeutic strategies. Breast cancer is currently the most common cancer globally, accounting for 12.5% of all new annual cancer cases worldwide. Although the rat model of mammary cancer chemically-induced is widely used to study this disease, there is a lack of standardization in procedures for cancer induction, sample collection, and analysis. Therefore, it is important to provide a practical guide for researchers aiming to work with this model to make the analysis of results more uniform. Thus, in this review, we provide the researchers with a detailed step-by-step guide to implement a rat model of mammary cancer, based on our wide experience in this field, to obtain the best results, maximum throughput of each experiment, and easy comparison among researches.
Keywords: carcinogenesis, guide, modeling, rodents, tips.
Funder
Fundação para a Ciência e a Tecnologia
Publisher
Veterinary World
Subject
General Veterinary
Reference46 articles.
1. Lee, Y.Y., Choi, M.C., Park, J.Y., Suh, D.H. and Kim, J.W. (2021) Major clinical research advances in gynecologic cancer in 2020. J. Gynecol. Oncol., 32(4): e53. 2. Ferlay, J., Colombet, M., Soerjomataram, I., Dyba, T., Randi, G., Bettio, M., Gavin, A., Visser, O. and Bray, F. (2018) Cancer incidence and mortality patterns in Europe: Estimates for 40 countries and 25 major cancers in 2018. Eur. J. Cancer, 103: 356–387. 3. World Health Organization, (WHO) Cancer. (2020) Available from: https://www.who.int/news-room/fact-sheets/detail/cancer Retrieved on 12-12-2022. 4. Baptista, C.V.J., Faustino-Rocha, A.I. and Oliveira, P.A. (2021) Animal models in pharmacology: A brief history awarding the Nobel prizes for physiology or medicine. Pharmacology, 106(7–8): 356–368. 5. Costa, E., Ferreira-Gonçalves, T., Cardoso, M., Coelho, J.M.P., Gaspar, M.M., Faísca, P., Ascensão, L., Cabrita, A.S., Reis, C.P. and Figueiredo, I.V. (2020) A step forward in breast cancer research: From a natural-like experimental model to a preliminary photothermal approach. Int. J. Mol. Sci., 21(24): 9681.
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