Minimally instrumented SHERLOCK (miSHERLOCK) for CRISPR-based point-of-care diagnosis of SARS-CoV-2 and emerging variants-Reference-Cited by-同舟云学术

Minimally instrumented SHERLOCK (miSHERLOCK) for CRISPR-based point-of-care diagnosis of SARS-CoV-2 and emerging variants

Published:2021-08-06 Issue:32 Volume:7 Page:
ISSN:2375-2548
Container-title:Science Advances
language:en
Short-container-title:Sci. Adv.

Author:

de Puig Helena¹²³^ORCID,Lee Rose A.¹⁴⁵⁶,Najjar Devora¹²⁷^ORCID,Tan Xiao¹²³⁶⁸,Soenksen Luis R.¹²⁹^ORCID,Angenent-Mari Nicolaas M.¹²^ORCID,Donghia Nina M.¹^ORCID,Weckman Nicole E.¹,Ory Audrey¹¹⁰,Ng Carlos F.¹,Nguyen Peter Q.¹^ORCID,Mao Angelo S.¹²^ORCID,Ferrante Thomas C.¹,Lansberry Geoffrey¹^ORCID,Sallum Hani¹,Niemi James¹,Collins James J.¹²³⁹¹⁰^ORCID

Affiliation:

1. Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.

2. Institute for Medical Engineering and Science, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

3. Infectious Disease and Microbiome Program, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.

4. Division of Infectious Diseases, Department of Pediatrics, Boston Children’s Hospital, Boston, MA 02115, USA.

5. Division of Infectious Diseases, Department of Medicine, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.

6. Harvard Medical School, Boston, MA 02115, USA.

7. MIT Media Lab, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

8. Division of Gastroenterology, Massachusetts General Hospital, Boston, MA 02114, USA.

9. Abdul Latif Jameel Clinic for Machine Learning in Health, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.

10. College of Arts and Sciences, Harvard University, Cambridge, MA 02138, USA.

Abstract

An integrated, low-cost, sample-to-answer, CRISPR-based diagnostic detects SARS-CoV-2 and variants from unprocessed saliva.

Funder

Hansjörg Wyss Institute for Biologically Inspired Engineering, Harvard University

MIT-Tata Center Fellowship

Harvard University Center for AIDS Research (CFAR)/NIH

Burroughs-Wellcome American Society of Tropical Medicine and Hygiene postdoctoral fellowship

American Gastroenterological Association Takeda Pharmaceutical Research Scholar Award in Inflammatory Bowel Disease

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference53 articles.

1. Synthetic biology in the clinic: engineering vaccines, diagnostics, and therapeutics

2. Point-of-Care Devices to Detect Zika and Other Emerging Viruses

3. CRISPR-based detection of SARS-CoV-2: A review from sample to result

4. Nucleic acid detection with CRISPR-Cas13a/C2c2

5. Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

Cited by 187 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Recent progress on rapid diagnosis of COVID-19 by point-of-care testing platforms;Chinese Chemical Letters;2024-03

2. A photocontrolled one-pot isothermal amplification and CRISPR-Cas12a assay for rapid detection of SARS-CoV-2 Omicron variants;Microbiology Spectrum;2024-02-06

3. Marine natural products and human immunity: novel biomedical resources for anti-infection of SARS-CoV-2 and related cardiovascular disease;Natural Products and Bioprospecting;2024-01-29

4. Peptide Nucleic Acid Clamp‐Assisted Photothermal Multiplexed Digital PCR for Identifying SARS‐CoV‐2 Variants of Concern;Advanced Science;2024-01-19

5. CRISPR‐based environmental DNA detection for a rare endangered estuarine species;Environmental DNA;2024-01-18