Identifying high-grade serous ovarian carcinoma–specific extracellular vesicles by polyketone-coated nanowires-Reference-Cited by-同舟云学术

Identifying high-grade serous ovarian carcinoma–specific extracellular vesicles by polyketone-coated nanowires

Published:2023-07-07 Issue:27 Volume:9 Page:
ISSN:2375-2548
Container-title:Science Advances
language:en
Short-container-title:Sci. Adv.

Author:

Yokoi Akira¹²³^ORCID,Ukai Mayu¹^ORCID,Yasui Takao⁴⁵⁶⁷^ORCID,Inokuma Yasuhide³⁶⁸⁹^ORCID,Hyeon-Deuk Kim⁶¹⁰^ORCID,Matsuzaki Juntaro¹¹^ORCID,Yoshida Kosuke¹²^ORCID,Kitagawa Masami¹¹²,Chattrairat Kunanon⁵^ORCID,Iida Mikiko⁵^ORCID,Shimada Taisuke⁵^ORCID,Manabe Yumehiro⁸,Chang I-Ya¹⁰^ORCID,Asano-Inami Eri¹¹²,Koya Yoshihiro¹¹²,Nawa Akihiro¹¹²,Nakamura Kae¹¹³^ORCID,Kiyono Tohru¹⁴^ORCID,Kato Tomoyasu¹⁵,Hirakawa Akihiko¹⁶,Yoshioka Yusuke¹⁷,Ochiya Takahiro¹⁷^ORCID,Hasegawa Takeshi¹⁸^ORCID,Baba Yoshinobu⁵⁷¹⁹^ORCID,Yamamoto Yusuke²⁰^ORCID,Kajiyama Hiroaki¹¹^ORCID

Affiliation:

1. Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.

2. Nagoya University Institute for Advanced Research, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.

3. Japan Science and Technology Agency (JST), FOREST, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.

4. Department of Life Science and Technology, Tokyo Institute of Technology, Nagatsuta 4259, Midori-ku, Yokohama 226-8501, Japan.

5. Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.

6. Japan Science and Technology Agency (JST), PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.

7. Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.

8. Division of Applied Chemistry, Faculty of Engineering, Hokkaido University, Kita 13 Nishi 8 Kita-ku, Sapporo, Hokkaido 060-8628, Japan.

9. Institute for Chemical Reaction Design and Discovery (WPI-ICReDD), Hokkaido University, Kita 21, Nishi 10, Kita-ku, Sapporo, Hokkaido 001-0021, Japan.

10. Department of Chemistry, Kyoto University, Yoshida-honmachi, Sakyo-ku, Kyoto 606-8502, Japan.

11. Division of Pharmacotherapeutics, Keio University Faculty of Pharmacy, 2-15-45 Mita, Minato-ku, Tokyo 108-8345, Japan.

12. Bell Research Center, Department of Obstetrics and Gynecology Collaborative Research, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.

13. Center for Low-Temperature Plasma Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.

14. Project for Prevention of HPV-related Cancer, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center, 6-5-1 Kashiwanoha, Kashiwa, Chiba 277-8577, Japan.

15. Department of Gynecologic Oncology, National Cancer Center Hospital, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan.

16. Department of Clinical Biostatistics, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8519, Japan.

17. Department of Molecular and Cellular Medicine, Institute of Medical Science, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan.

18. Institute for Chemical Research, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.

19. Institute of Quantum Life Science, National Institutes for Quantum and Radiological Science and Technology, 4-9-1 Kanagawa, Inage-ku, Chiba, Chiba 263-8555, Japan.

20. Laboratory of Integrative Oncology, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan.

Abstract

Cancer cell–derived extracellular vesicles (EVs) have unique protein profiles, making them promising targets as disease biomarkers. High-grade serous ovarian carcinoma (HGSOC) is the deadly subtype of epithelial ovarian cancer, and we aimed to identify HGSOC-specific membrane proteins. Small EVs (sEVs) and medium/large EVs (m/lEVs) from cell lines or patient serum and ascites were analyzed by LC-MS/MS, revealing that both EV subtypes had unique proteomic characteristics. Multivalidation steps identified FRα, Claudin-3, and TACSTD2 as HGSOC-specific sEV proteins, but m/lEV-associated candidates were not identified. In addition, for using a simple-to-use microfluidic device for EV isolation, polyketone-coated nanowires (pNWs) were developed, which efficiently purify sEVs from biofluids. Multiplexed array assays of sEVs isolated by pNW showed specific detectability in cancer patients and predicted clinical status. In summary, the HGSOC-specific marker detection by pNW are a promising platform as clinical biomarkers, and these insights provide detailed proteomic aspects of diverse EVs in HGSOC patients.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Link

https://www.science.org/doi/pdf/10.1126/sciadv.ade6958

Reference56 articles.

1. Biogenesis, Secretion, and Intercellular Interactions of Exosomes and Other Extracellular Vesicles

2. Exosomes and extracellular vesicles: Rethinking the essential values in cancer biology

3. Circulating Extracellular Vesicles in Human Disease

4. Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen

5. MIFlowCyt‐EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments

Cited by 3 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. An electrochemical biosensor designed with entropy-driven autocatalytic DNA circuits for sensitive detection of ovarian cancer-derived exosomes;Biosensors and Bioelectronics;2024-04

2. Tandem SERS and MS/MS Profiling of Plasma Extracellular Vesicles for Early Ovarian Cancer Biomarker Discovery;ACS Sensors;2024-01-12

3. Signal transduction pathways alter the molecular cargo of extracellular vesicles: implications in regenerative medicine;Regenerative Medicine;2023-12