Bright and sensitive red voltage indicators for imaging action potentials in brain slices and pancreatic islets-Reference-Cited by-同舟云学术

Bright and sensitive red voltage indicators for imaging action potentials in brain slices and pancreatic islets

Published:2023-11-24 Issue:47 Volume:9 Page:
ISSN:2375-2548
Container-title:Science Advances
language:en
Short-container-title:Sci. Adv.

Author:

Han Yi¹^ORCID,Yang Junqi²^ORCID,Li Yuan³⁴,Chen Yu¹³^ORCID,Ren Huixia³⁵^ORCID,Ding Ran⁶,Qian Weiran⁷,Ren Keyuan¹,Xie Beichen³,Deng Mengying⁸,Xiao Yinghan⁸^ORCID,Chu Jun⁸,Zou Peng¹²³⁴^ORCID

Affiliation:

1. College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, PKU-IDG/McGovern Institute for Brain Research, Peking University, Beijing 100871, China.

2. Peking University–Tsinghua University–National Institute of Biological Sciences Joint Graduate Program, School of Life Sciences, Peking University, Beijing 100871, China.

3. Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.

4. Chinese Institute for Brain Research (CIBR), Beijing 102206, China.

5. Center for Quantitative Biology, Peking University, Beijing 100871, China.

6. Institute for Translational Neuroscience of the Second Affiliated Hospital of Nantong University, Center for Neural Developmental and Degenerative Research of Nantong University, Nantong 226001, China.

7. Institute of Molecular Medicine, College of Future Technology, Peking University, Beijing 100871, China.

8. Research Center for Biomedical Optics and Molecular Imaging, Shenzhen Key Laboratory for Molecular Imaging, Guangdong Provincial Key Laboratory of Biomedical Optical Imaging Technology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.

Abstract

Genetically encoded voltage indicators (GEVIs) allow the direct visualization of cellular membrane potential at the millisecond time scale. Among these, red-emitting GEVIs have been reported to support multichannel recordings and manipulation of cellular activities with reduced autofluorescence background. However, the limited sensitivity and dimness of existing red GEVIs have restricted their applications in neuroscience. Here, we report a pair of red-shifted opsin-based GEVIs, Cepheid1b and Cepheid1s, with improved dynamic range, brightness, and photostability. The improved dynamic range is achieved by a rational design to raise the electrochromic Förster resonance energy transfer efficiency, and the higher brightness and photostability are approached with separately engineered red fluorescent proteins. With Cepheid1 indicators, we recorded complex firings and subthreshold activities of neurons on acute brain slices and observed heterogeneity in the voltage‑calcium coupling on pancreatic islets. Overall, Cepheid1 indicators provide a strong tool to investigate excitable cells in various sophisticated biological systems.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Link

https://www.science.org/doi/pdf/10.1126/sciadv.adi4208

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