XYZeq: Spatially resolved single-cell RNA sequencing reveals expression heterogeneity in the tumor microenvironment-Reference-Cited by-同舟云学术

XYZeq: Spatially resolved single-cell RNA sequencing reveals expression heterogeneity in the tumor microenvironment

Published:2021-04-23 Issue:17 Volume:7 Page:
ISSN:2375-2548
Container-title:Science Advances
language:en
Short-container-title:Sci. Adv.

Author:

Lee Youjin¹²³⁴,Bogdanoff Derek⁵⁶,Wang Yutong⁷⁸^ORCID,Hartoularos George C.⁹¹⁰¹¹^ORCID,Woo Jonathan M.¹²³⁴,Mowery Cody T.¹²³⁴¹²^ORCID,Nisonoff Hunter M.⁸^ORCID,Lee David S.³¹⁰¹¹^ORCID,Sun Yang³¹⁰¹¹^ORCID,Lee James¹³^ORCID,Mehdizadeh Sadaf²^ORCID,Cantlon Joshua¹⁴^ORCID,Shifrut Eric¹²³⁴^ORCID,Ngyuen David N.¹²³⁴¹⁵^ORCID,Roth Theodore L.¹²³¹²¹⁶^ORCID,Song Yun S.¹⁷¹⁸¹⁹^ORCID,Marson Alexander¹²³⁴¹⁵^ORCID,Chow Eric D.⁵⁶^ORCID,Ye Chun Jimmie¹⁰¹⁹²⁰¹¹²¹^ORCID

Affiliation:

1. Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA.

2. Diabetes Center, University of California, San Francisco, San Francisco, CA 94143, USA.

3. Innovative Genomics Institute, University of California, Berkeley, Berkeley, CA 94720, USA.

4. J. David Gladstone Institutes, San Francisco, CA 94158, USA.

5. Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94158, USA.

6. Center for Advanced Technology, University of California, San Francisco, San Francisco, CA 94158, USA.

7. Graduate Group in Biostatistics, University of California, Berkeley, CA 94720, USA.

8. Center for Computational Biology, University of California, Berkeley, CA 94720, USA.

9. Graduate Program in Biological and Medical Informatics, University of California, San Francisco, San Francisco, CA 94158, USA.

10. Division of Rheumatology, Department of Medicine, University of California, San Francisco, CA 94143, USA.

11. Institute for Human Genetics, University of California, San Francisco, San Francisco, CA 94143, USA.

12. Medical Scientist Training Program, University of California, San Francisco, San Francisco, CA 94143, USA.

13. Division of Hematology and Oncology, University of California, San Francisco, San Francisco, CA 94143, USA.

14. Scienion AG, Volmerstrasse 7b, 12489 Berlin, Germany.

15. Department of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.

16. Biomedical Sciences Graduate Program, University of California, San Francisco, San Francisco, CA 94143, USA.

17. Computer Science Division, University of California, Berkeley, CA 94720, USA.

18. Department of Statistics, University of California, Berkeley, CA 94720, USA.

19. Chan Zuckerberg Biohub, San Francisco, CA 94158, USA.

20. Parker Institute for Cancer Immunotherapy, University of California, San Francisco, San Francisco, CA 94129, USA.

21. Gladstone–UCSF Institute of Genomic Immunology, San Francisco, CA 94158, USA.

Abstract

XYZeq is a novel scalable platform that directly encodes spatial location from tissue into single-cell RNA sequencing libraries.

Funder

National Institutes of Health

T32 Ruth Kirschstein Fellowship

University of California, San Francisco

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference51 articles.

1. Single-cell RNA-seq highlights intratumoral heterogeneity in primary glioblastoma

2. Single-Cell Transcriptomic Analysis of Primary and Metastatic Tumor Ecosystems in Head and Neck Cancer

3. Comparative Analysis of Single-Cell RNA Sequencing Methods

4. Single-Cell Multiomics: Multiple Measurements from Single Cells

5. Single-Cell RNA Sequencing in Cancer: Lessons Learned and Emerging Challenges

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