Native phasing of x-ray free-electron laser data for a G protein–coupled receptor

Author:

Batyuk Alexander1ORCID,Galli Lorenzo2,Ishchenko Andrii34,Han Gye Won34ORCID,Gati Cornelius2,Popov Petr A.456,Lee Ming-Yue34ORCID,Stauch Benjamin34ORCID,White Thomas A.2ORCID,Barty Anton2,Aquila Andrew7,Hunter Mark S.7ORCID,Liang Mengning7,Boutet Sébastien7,Pu Mengchen8ORCID,Liu Zhi-jie89ORCID,Nelson Garrett10,James Daniel10ORCID,Li Chufeng10ORCID,Zhao Yun10ORCID,Spence John C. H.10,Liu Wei11,Fromme Petra11,Katritch Vsevolod346,Weierstall Uwe10,Stevens Raymond C.3469,Cherezov Vadim345612

Affiliation:

1. Department of Biochemistry, University of Zürich, Winterthurerstrasse 190, 8057 Zürich, Switzerland.

2. Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607 Hamburg, Germany.

3. Department of Chemistry, University of Southern California, Los Angeles, CA 90089, USA.

4. Bridge Institute, University of Southern California, Los Angeles, CA 90089, USA.

5. Moscow Institute of Physics and Technology, Dolgoprudny 141700, Russia.

6. Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089, USA.

7. Linac Coherent Light Source, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025, USA.

8. National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.

9. iHuman Institute, ShanghaiTech University, Shanghai 201210, China.

10. Department of Physics, Arizona State University, Tempe, AZ 85287, USA.

11. Center for Applied Structural Discovery at the Biodesign Institute, School of Molecular Sciences, Arizona State University, 727 East Tyler Street, Tempe, AZ 85287, USA.

12. Department of Physics and Astronomy, University of Southern California, Los Angeles, CA 90089, USA.

Abstract

Anomalous signal from sulfur atoms present in most proteins was used for de novo phasing of XFEL data and solving a GPCR structure.

Funder

National Institute of General Medical Sciences

Division of Biological Infrastructure

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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