Requirement of Phospholipase Cδ4 for the Zona Pellucida-Induced Acrosome Reaction

Author:

Fukami Kiyoko1,Nakao Kazuki2,Inoue Takafumi3,Kataoka Yuki4,Kurokawa Manabu5,Fissore Rafael A.5,Nakamura Kenji2,Katsuki Motoya2,Mikoshiba Katsuhiko36,Yoshida Nobuaki4,Takenawa Tadaomi1

Affiliation:

1. Department of Biochemistry,

2. Division of DNA Biology and Embryo Engineering, Center for Experimental Medicine,

3. Department of Neurobiology,

4. Division of Gene Expression and Regulation, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8039, Japan.

5. Department of Veterinary and Animal Sciences, Paige Laboratory, University of Massachusetts, Amherst, MA 01003, USA.

6. Brain Science Institute, Riken, Hirosawa, Wako, Saitama 351-0198, Japan.

Abstract

Several phospholipase C (PLC) isoforms have been found in male and female mammalian gametes, and splicing isoforms of PLCδ4 are predominantly expressed in testis. Here we report that male mice in which the PLCδ4 gene had been disrupted either produced few small litters or were sterile. In vitro fertilization studies showed that insemination with PLCδ4 −/− sperm resulted in significantly fewer eggs becoming activated and that the calcium transients associated with fertilization were absent or delayed. PLCδ4 −/− sperm were unable to initiate the acrosome reaction, an exocytotic event required for fertilization and induced by interaction with the egg coat, the zona pellucida. These data demonstrate that PLCδ4 functions in the acrosome reaction that is induced by the zona pellucida during mammalian fertilization.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

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