Role of Formins in Actin Assembly: Nucleation and Barbed-End Association-Reference-Cited by-同舟云学术

Role of Formins in Actin Assembly: Nucleation and Barbed-End Association

Published:2002-07-26 Issue:5581 Volume:297 Page:612-615
ISSN:0036-8075
Container-title:Science
language:en
Short-container-title:Science

Author:

Pruyne David¹,Evangelista Marie²,Yang Changsong³,Bi Erfei⁴,Zigmond Sally³,Bretscher Anthony¹,Boone Charles²⁵

Affiliation:

1. Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.

2. Department of Biology, Queen's University, Kingston, Ontario, K7L 3N6 Canada.

3. Biology Department, University of Pennsylvania, Philadelphia, PA 19104–6018, USA.

4. Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.

5. Banting and Best Department of Medical Research and Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario, M5G 1L6 Canada.

Abstract

Nucleation of branched actin filaments by the Arp2/3 complex is a conserved process in eukaryotic cells, yet the source of unbranched actin filaments has remained obscure. In yeast, formins stimulate assembly of actin cables independently of Arp2/3. Here, the conserved core of formin homology domains 1 and 2 of Bni1p (Bni1pFH1FH2) was found to nucleate unbranched actin filaments in vitro . Bni1pFH2 provided the minimal region sufficient for nucleation. Unique among actin nucleators, Bni1pFH1FH2 remained associated with the growing barbed ends of filaments. This combination of properties suggests a direct role for formins in regulating nucleation and polarization of unbranched filamentous actin structures.

Publisher

American Association for the Advancement of Science (AAAS)

Subject

Multidisciplinary

Reference34 articles.

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